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Highly dynamic... ...immunoglobulin genesMetaSystems is introducing two new probes, XL 2p11 IGK BA and XL 22q11 IGL BA, replacing XL IGK and XL IGL, respectively. Both probes have a break apart design, ensuring the detection of IGL or IGK rearrangements independent of the translocation partner. XL 2p11 IGK BA and XL 22q11 IGL BA are optimized to obtain brilliant signals and an excellent signal to background ratio. The immunoglobulin (IG) genes for the kappa light chain at 2p12 (IGK), the lambda light chain at 22q11 (IGL) and the heavy chain at 14q32 (IGH) are recurrently involved in the development of Non-Hodgkin lymphomas. By far most frequently involved is IGH with more than 30 partner genes, less frequently IGK and IGL. IG-translocations are leading to juxtaposition of proto-oncogenes with IG enhancer sequences resulting in overexpression of the respective oncogene. FISH break-apart assays are valuable tools for the detection of IG light chains rearrangements independent of the translocation partner. Furthermore, double translocations have been described which are difficult to detect by PCR-based methods.
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XL 2p11 IGK BAClinical Applications: LymphomaXL 2p11 IGK BA is designed as a break apart probe. The orange labeled probe hybridizes to the ´IGKV distal´ region at 2p11.2, the green labeled probe hybridizes distal to the ´IGKV proximal´, IGKJ and IGKC region. Besides 8q24 (MYC), other translocation partners for IGK, as chromosomal regions 7q21, 16q24 and 18q21 (BCL2), are known.
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Upper image: Signal pattern in normal cells hybridized with XL 2p11 IGK BA or XL 22q11 IGL BA: Two green-orange colocalization-fusion signals (2GO). Lower image: Signal pattern in typical aberrant cells hybridized with XL 2p11 IGK BA or XL 22q11 IGL BA: One green-orange colocalization-fusion signal (1GO), one separate green (1G) and orange (1O) signal each resulting from a chromosome break in the respective locus.
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XL 22q11 IGL BAClinical Applications: LymphomaXL 22q11 IGL BA is designed as a break apart probe. The orange labeled probe hybridizes proximal to the breakpoint in the IGLV gene region at 22q11, the green labeled probe hybridizes distal to the breakpoint. Besides 8q24 (MYC), other translocation partners for IGL, as chromosomal regions 2p13-14, 3q27 (BCL6), 4q13 and 16p12, are known.
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