Human Multicolor FISH Probe

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(12 Tests)



24 painting probes specific for the 24 different human chromosomes, labeled with different fluorochromes. The excitation/emission spectra are comparable to the common aqua, green, orange, red and near infrared fluorochromes.

Also available as 120µl (2x60µl) D-0125-120-DI and as 600µl (10x60µl) D-0125-600-DI.

Probe map details based on UCSC Genome Browser GRCh37/hg19. Map components not to scale.

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Clinical Details

The principle of mFISH is based on combinatorial labeling of whole chromosome paints. For each chromosome the different fluorochromes are mixed resulting in a unique color combination (see labeling scheme). The analysis is carried out after capturing grey scale images of each fluorochrome. For best separation of the different colors the filter sets used need to be highly selective assuring that cross-talk is reduced to a minimum. Multicolor FISH Analysis with MetaSystems‘ Isis supports the analysis by applying appropriate algorithms which leads to pseudo coloring of the different chromosome classes (color combinations). Each chromosome class will be displayed in a unique color. Translocations show up as color changes on individual chromosomes.

Clinical Applications

  • Compl. CA
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24XCyte hybridized to lymphocytes. Multiple translocations can be identified by conjunction of differently labeled chromosomal fragments.

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  • Speicher MR et al (1996) Nat Genet 12:368-375
  • Fauth C and Speicher MR (2001) Cytogenet Cell Genet 93:1-10
  • Schoch C et al (2002) Genes Chromosomes Cancer 35:20-29