The MetaSystems' TissueFISH Pretreatment Kit contains reagents and solutions to process formalin-fixed paraffin-embedded tissue sections for FISH assays. Reagents provided are: 5x 5 ml 10x Pretreatment Buffer, 200 ml Protease Buffer, 500 µl Protease Stock Solution.

The MetaSystems' TissueFISH Pretreatment Kit provides reagents which help to unmask formalin-fixed paraffin-embedded tissue cells to allow FISH. The first step is permeabilization which is thought to reverse protein cross-linking caused by formalin fixation. In a second step cells are digested by a protease to facilitate penetration of the fluorescent probe. Processing tissue slides using the MetaSystems' TissueFISH Pretreatment Kit will take less than 90 minutes.

The kit comes in two parts:
Part 1: Pretreatment reagents, stored at room temperature
Part 2: Protease, to be stored at 2°C to 8°C upon arrival

TissueFISH Pretreatment Kit (Part 1 and 2)

• Fioretos et al (1999) Blood 94:225-232
• Srinivasan et al (2002) Am J Pathol 161:1961-1971
• Chin et al (2003) Mol Pathol 56:275-279