TissueFISH Pretreatment Kit

General Reagents

Order Number
D-0905-025-TF
Labels
Chromosomes

IVDR Certification

MetaSystems Probes has already certified a large part of its portfolio, according to IVDR. For organizational reasons, we currently provide only the IVDD product.

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Product Description

The MetaSystems' TissueFISH Pretreatment Kit contains reagents and solutions to process formalin-fixed paraffin-embedded tissue sections for FISH assays. Reagents provided are: 5x 5 ml 10x Pretreatment Buffer, 200 ml Protease Buffer, 500 µl Protease Stock Solution.

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Clinical Details

The MetaSystems' TissueFISH Pretreatment Kit provides reagents which help to unmask formalin-fixed paraffin-embedded tissue cells to allow FISH. The first step is permeabilization which is thought to reverse protein cross-linking caused by formalin fixation. In a second step cells are digested by a protease to facilitate penetration of the fluorescent probe. Processing tissue slides using the MetaSystems' TissueFISH Pretreatment Kit will take less than 90 minutes.

The kit comes in two parts:
Part 1: Pretreatment reagents, stored at room temperature
Part 2: Protease, to be stored at 2°C to 8°C upon arrival

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Images

TissueFISH Pretreatment Kit

TissueFISH Pretreatment Kit (Part 1 and 2)

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Literature

  • Fioretos et al (1999) Blood 94:225-232
  • Srinivasan et al (2002) Am J Pathol 161:1961-1971
  • Chin et al (2003) Mol Pathol 56:275-279

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News

IVDR Certification

MetaSystems Probes has received IVDR certification for our initial 26 fluorescence in situ hybridization (FISH) probes from the notified body, BSI. Achieving this milestone was not without its challenges, and we are delighted to have accomplished IVDR certification for this probe set at an early stage.

IVDR Certification

New Probes Catalog is Online!

 

MetaSystems Probes is pleased to announce the new catalog including new and innovative products and supporting information. A PDF version of the new catalog is available for download here.

New Probes Catalog is Online!