About 100 guests from 36 countries met on the XVIII. MetaSystems Distributor Meeting (DM) in November to exchange experiences and to get to know new trends and developments at MetaSystems.
TissueFISH Pretreatment Kit
- Order Number
The MetaSystems' TissueFISH Pretreatment Kit contains reagents and solutions to process formalin-fixed paraffin-embedded tissue sections for FISH assays. Reagents provided are: 5x 5 ml 10x Pretreatment Buffer, 200 ml Protease Buffer, 500 µl Protease Stock Solution.
The MetaSystems' TissueFISH Pretreatment Kit provides reagents which help to unmask formalin-fixed paraffin-embedded tissue cells to allow FISH. The first step is permeabilization which is thought to reverse protein cross-linking caused by formalin fixation. In a second step cells are digested by a protease to facilitate penetration of the fluorescent probe. Processing tissue slides using the MetaSystems' TissueFISH Pretreatment Kit will take less than 90 minutes.
The kit comes in two parts:
Part 1: Pretreatment reagents, stored at room temperature
Part 2: Protease, to be stored at 2°C to 8°C upon arrival
- Fioretos et al (1999) Blood 94:225-232
- Srinivasan et al (2002) Am J Pathol 161:1961-1971
- Chin et al (2003) Mol Pathol 56:275-279