The MetaSystems' TissueFISH Pretreatment Kit provides reagents which help to unmask formalin-fixed paraffin-embedded tissue cells to allow FISH. The first step is permeabilization which is thought to reverse protein cross-linking caused by formalin fixation. In a second step cells are digested by a protease to facilitate penetration of the fluorescent probe. Processing tissue slides using the MetaSystems' TissueFISH Pretreatment Kit will take less than 90 minutes.
The kit comes in two parts:
Part 1: Pretreatment reagents, stored at room temperature
Part 2: Protease, to be stored at 2°C to 8°C upon arrival