MetaSystems proudly announces the opening of our first subsidiary in the Southern hemisphere. The new office is located in Buenos Aires, Argentina and bears the name MetaSystems Latinoamérica SAS (MetaSystems LatAm). The foundation has been the response to the growing demand on technical knowledge and 'know how' experienced in the last years all over the Latin American territories. MetaSystems LatAm takes care for the organization of sales, support, and customer relation activities in all countries in Latin America. It will manage distributor companies and contacts in the region, and it will facilitate communication between end-users, distributors and headquarters in Germany.
TissueFISH Pretreatment Kit
- Order Number
The MetaSystems' TissueFISH Pretreatment Kit contains reagents and solutions to process formalin-fixed, paraffin-embedded tissue sections for FISH assays. Reagents provided are: 5x 5 ml 10x Pretreatment Buffer, 200 ml Protease Buffer, 500 µl Protease Stock Solution.
The MetaSystems' TissueFISH Pretreatment Kit provides reagents which help to unmask formalin-fixed, paraffin-embedded tissue cells to allow FISH. The first step is permeabilization which is thought to reverse protein cross-linking caused by formalin fixation. In a second step cells are digested by a protease to facilitate penetration of the fluorescent probe. Processing tissue slides using the MetaSystems' TissueFISH Pretreatment Kit will take less than 90 minutes.
The kit comes in two parts:
Part 1 Pretreatment reagents, stored at room temperature
Part 2 Protease, to be stored at 2°C to 8°C upon arrival
- Fioretos et al (1999) Blood 94:225-232
- Srinivasan et al (2002) Am J Pathol 161:1961-1971
- Chin et al (2003) Mol Pathol 56:275-279