Fluorescence in situ hybridization has become an essential detection assay in today´s routine diagnostics. However, long hybridization times of many hours to overnight are still a restrictive factor. We have refined the production process of our FISH probes to reduce background and artefacts and to improve the signal to noise ratio, particularly in short-time hybridization. Since mid-2015, one hour hybridization on lymphocytes is an integral part of quality control for all XCyting locus-specific probes at our manufacturing facility.
Translocation/Dual Fusion Probe
- Order Number
- Package Size
- 100 µl
Chromosomal translocations affecting the IGH locus are recurrent in many types of lymphomas. The malignant transformation works via the juxtaposition of oncogenes next to regulatory sequences of the immunoglobulin locus.
The t(8;14)(q24;q32) MYC/IGH is the hallmark translocation of human Burkitt lymphoma where it accounts for about 85% of cases. The same translocation can be present in almost 5% of ALL patients and in diffuse large B-cell lymphomas. Although this aberration is often described to indicate a good prognosis, it can be an indicator of bad prognosis if associated with other factors, such as second aberrations like t(14;18) or BCL6 rearrangements.
- Non-Hodgkin Lymphomas (NHL)
- Acute Lymphoblastic Leukemia (ALL)
Two green (2G) and two orange signals (2O).
Aberrant Cell (typical results):
One green (1G), one orange (1O), and two green-orange fusion signals (2GO) resulting from a reciprocal translocation between the relevant loci.
- Siebert et al (1998) Blood 91:984-990
- Hummel et al (2006) N Engl J Med 354:2419-30
- Boerma et al (2009) Leukemia 23:225-234