Fluorescence in situ hybridization has become an essential detection assay in today´s routine diagnostics. However, long hybridization times of many hours to overnight are still a restrictive factor. We have refined the production process of our FISH probes to reduce background and artefacts and to improve the signal to noise ratio, particularly in short-time hybridization. Since mid-2015, one hour hybridization on lymphocytes is an integral part of quality control for all XCyting locus-specific probes at our manufacturing facility.
Break Apart Probe
- Order Number
- Package Size
- 100 µl
Primary karyotypic changes in lymphoid neoplasms commonly juxtapose oncogenes to the potent transcriptional enhancers associated with IG and TCR loci in B and T cells, respectively, often resulting in elevated levels of protein expression and loss of normal mechanisms of control. Less commonly, fusion genes are created that encode novel hybrid proteins.
Variant translocations involving the IG kappa (IGK) locus in 2p11.2 or the IG lambda (IGL) locus in 22q11 occur recurrently in B-cell neoplasias. The different translocations involving immunoglobulin genes have a diagnostic value and can be used to monitor the clinical course of the disease.
- Non-Hodgkin Lymphomas (NHL)
Two green-orange (2GO) fusion signals.
Aberrant Cell (typical results):
One green-orange fusion signal (1GO) and one green (1G), one orange (1O) indicating a chromosome break in the IGK locus.
- Martin-Subero et al (2002) Int J Cancer 98:470-474
- Wlodarska et al (2004) Leukemia 10:1705-1710
- Einerson et al (2006) Leukemia 20:1790-1799