XL TET2

Deletion Probe

Order Number
D-5038-100-OG
Package Size
100 µl
Labels
  
Chromosome
4

Description

XL TET2

The XL TET2 locus-specific probe detects deletions in 4q24. This probe is labeled in orange and extends from marker RH43141 proximal to RH69608 distal of the TET2 gene. A green labeled probe hybridizes to 4q12, functioning as a reference probe.

Clinical Details

The myelodysplastic syndromes and myeloproliferative disorders are associated with deregulated production of myeloid cells. According to WHO classification (2008) cytogenetic aberrations are observed in about 50 % of MDS cases. The most common aberrations are 5q-, 7/7q-, trisomy 8, del(20q), and inv(3) or t(3;3).

A minimally deleted region on chromosome 4q24 is described in subgroups of patients with myelodysplastic syndromes and acute myeloid leukemia (AML). The region encompasses the TET2 gene. The frequency of TET2 mutations in unselected patients was 19 % (15 of 81 patients) with myelodysplastic syndromes, 12 % (24 of 198 patients) with myeloproliferative disorders, 24 % (5 of 21 patients) with secondary AML, and 22 % (2 of 9 patients) with chronic myelomonocytic leukemia.

Clinical Applications

  • Myelodysplastic Syndrome (MDS)
  • Acute Myelogenous Leukemia (AML)
  • Chronic Myelogenous Leukemia (CML)

Images

XL TET2

XL TET2 hybridized to lymphocytes. One partial normal metaphase and two normal interphases are shown.

Expected Patterns

Expected Pattern 1

Normal Cell:
Two green (2G) and two orange (2O) signals.

Expected Pattern 2

Aberrant Cell (typical results):
Two green (2G) and one orange (1O) signal, indicating a deletion of TET2 (4q24).

Literature

  • Jankowska et al (2009) Blood 113:6403-6410
  • Delhommeau et al (2009) N Engl J Med 360:2289-2301
  • Flach et al (2010) Haematologica 95:518-519

Downloads

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