Fluorescence in situ hybridization has become an essential detection assay in today´s routine diagnostics. However, long hybridization times of many hours to overnight are still a restrictive factor. We have refined the production process of our FISH probes to reduce background and artefacts and to improve the signal to noise ratio, particularly in short-time hybridization. Since mid-2015, one hour hybridization on lymphocytes is an integral part of quality control for all XCyting locus-specific probes at our manufacturing facility.
- Order Number
- Package Size
- 100 µl
The myelodysplastic syndromes and myeloproliferative disorders are associated with deregulated production of myeloid cells. According to WHO classification (2008) cytogenetic aberrations are observed in about 50 % of MDS cases. The most common aberrations are 5q-, 7/7q-, trisomy 8, del(20q), and inv(3) or t(3;3).
A minimally deleted region on chromosome 4q24 is described in subgroups of patients with myelodysplastic syndromes and acute myeloid leukemia (AML). The region encompasses the TET2 gene. The frequency of TET2 mutations in unselected patients was 19 % (15 of 81 patients) with myelodysplastic syndromes, 12 % (24 of 198 patients) with myeloproliferative disorders, 24 % (5 of 21 patients) with secondary AML, and 22 % (2 of 9 patients) with chronic myelomonocytic leukemia.
- Myelodysplastic Syndrome (MDS)
- Acute Myelogenous Leukemia (AML)
- Chronic Myelogenous Leukemia (CML)
Two green (2G) and two orange (2O) signals.
Aberrant Cell (typical results):
Two green (2G) and one orange (1O) signal, indicating a deletion of TET2 (4q24).
- Jankowska et al (2009) Blood 113:6403-6410
- Delhommeau et al (2009) N Engl J Med 360:2289-2301
- Flach et al (2010) Haematologica 95:518-519