Fluorescence in situ hybridization has become an essential detection assay in today´s routine diagnostics. However, long hybridization times of many hours to overnight are still a restrictive factor. We have refined the production process of our FISH probes to reduce background and artefacts and to improve the signal to noise ratio, particularly in short-time hybridization. Since mid-2015, one hour hybridization on lymphocytes is an integral part of quality control for all XCyting locus-specific probes at our manufacturing facility.
- Order Number
- Package Size
- 100 µl
The myelodysplastic syndromes and myeloproliferative disorders are associated with deregulated production of myeloid cells. According to WHO classification (2008) cytogenetic aberrations are observed in about 50 % of MDS cases. The most common aberrations are 5q-, 7/7q-, trisomy 8, del(20q), and inv(3) or t(3;3).
A chromosome 20q deletion is associated with about 5 % of primary MDS. The majority of cases has an interstitial deletion between 20q11.2 and q13.3. Isochromosome of the long arm of chromosome 20 with loss of interstitial material [ider(20q)] is a variant of deletion of chromosome 20q. Amplification of genes included in retained regions associated with loss of tumor suppressor genes in deleted regions could explain cell tumor progression and possibly the less favorable prognosis of ider(20q) compared with del(20q).
- Myelodysplastic Syndrome (MDS)
- Acute Myelogenous Leukemia (AML)
Normal Cell: Two green (2G) and two orange (2O) signals.
Aberrant Cell (typical results):
Two green (2G) and one orange (1O) signal, indicating a deletion in 20q12.
- Saunders et al (2005) Cancer Genet Cytogen 156:154-157
- Smoley et al (2006) Cancer Genet Cytogenet 173:144-149
- Douet-Gilbert et al (2008) Br J Haematol 143:716-720