Fluorescence in situ hybridization has become an essential detection assay in today´s routine diagnostics. However, long hybridization times of many hours to overnight are still a restrictive factor. We have refined the production process of our FISH probes to reduce background and artefacts and to improve the signal to noise ratio, particularly in short-time hybridization. Since mid-2015, one hour hybridization on lymphocytes is an integral part of quality control for all XCyting locus-specific probes at our manufacturing facility.
- Order Number
- Package Size
- 100 µl
The XL 7q22/7q36 locus-specific probe detects deletions in the long arm of chromosome 7. The orange labeled probe hybridizes to a specific region at 7q22 including the KMT2E (formerly MLL5) gene. The green labeled probe hybridizes specifically to 7q36 and includes the EZH2 gene. A blue (aqua) labeled probe which hybridizes to the centromere of chromosome 7 functions as a reference probe.
The myelodysplastic syndromes and myeloproliferative disorders are associated with deregulated production of myeloid cells. According to WHO classification (2008) cytogenetic aberrations are observed in about 50 % of MDS cases. The most common aberrations are 5q-, 7/7q-, trisomy 8, del(20q), and inv(3) or t(3;3).
Loss of chromosome 7 (-7) or deletion of the long arm (7q-) are recurring chromosome abnormalities in myeloid leukemias. The association of -7/7q- with myeloid leukemia suggests that certain regions contain tumor suppressor gene(s), whose loss of function contribute to leukemic transformation or tumor progression. Two critical regions have been identified, one in band 7q22 including the KMT2E gene and another in bands 7q35-q36 comprising the EZH2 gene.
- Myelodysplastic Syndrome (MDS)
- Acute Myelogenous Leukemia (AML)
Normal Cell: Two green (2G), two orange (2O), and two blue (2B) signals.
Aberrant Cell (typical results):
Two green (2G), one orange (1O), and two blue (2B) signals indicating the deletion of a 7q22 locus or one green (1G), two orange (2O), and two blue (2B) signals, indicating the deletion of a 7q36 locus.
Aberrant Cell (typical results): One green (1G), one orange (1O), and two blue (2B) signals indicating the deletion of a 7q22 locus and of a 7q36 locus.
Aberrant Cell (typical results): One green (1G), one orange (1O), and one blue (1B) signal indicating a monosomy 7.
- Le Beau et al (1996) Blood 88:1930-1935
- Emerling et al (2002) Oncogene 21:4849 - 4854
- De Weer et al (2010) PLoS One 13:e8676