XL CDKN2A

Deletion Probe

Order Number
D-5053-100-OG
Package Size
100 µl
Labels
  
Chromosome
9

Description

XL CDKN2A

The XL CDKN2A locus-specific probe detects deletions in band 9p21. This probe is labeled in orange and hybridizes to CDKN2A (p16) as well as CDKN2B (p15). The green labeled probe hybridizes to the centromere of chromosome 9 and functions as a reference probe.

Clinical Details

Acute lymphoblastic leukemia (ALL) is the most common childhood cancer type. T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive and quickly progressing type of ALL affecting T-lymphocytes. Inactivation of the tumor suppressor genes CDKN2A/2B, located at chromosomal region 9p21, is a significant event in the development of T-ALL and other cancer types. Principally, loss of function might happen by deletion, methylation of promotor regions or mutations, whereas deletion of chromosomal region 9p21 seems to be the predominant mechanism. CDKN2A/2B deletions can be detected in about 60% of pediatric and about 50% of adult T-ALL cases. Most deletions are within the resolution of the FISH technique. Genetic alterations of the 9p21 locus result in loss of regulation of the cell cycle which is critical to cancer development.

Clinical Applications

  • Acute Lymphoblastic Leukemia (ALL)

Images

XL CDKN2A

XL CDKN2A hybridized to bone marrow cells. Two normal cells and several cells with two green signals are shown indicating a homozygous deletion of the CDKN2A.

Expected Patterns

Expected Pattern 1

Normal Cell:
Two green (2G) and two orange (2O) signals.

Expected Pattern 2

Aberrant Cell (typical results):
Two green (2G) and one orange (1O) signal, indicating the deletion of the CDKN2A (p16) at 9p21.

Expected Pattern 3

Aberrant Cell (typical results): Two green (2G) and no orange (no O) signal, indicating a homozygous deletion of the CDKN2A (p16) at 9p21.

Literature

  • Novara et al (2009) Human Genet 126:511-520
  • Sulong et al (2009) Blood 113:100-107
  • Girardi et al (2017) Blood 129:1113-1123

Downloads

Order Now or Request Assistance

Contact Us »

Contact MetaSystems directly or find your local MetaSystems partner.