The XL MECOM 3q26 probe is designed as a break apart probe. Its orange labeled part hybridizes proximal to the MECOM gene locus at 3q26, the green labeled probe hybridizes to the distal gene region. MECOM stands for 'MDS1 and EVI1 complex locus'.
The myelodysplastic syndromes and myeloproliferative disorders are associated with deregulated production of myeloid cells. According to WHO classification (2008) cytogenetic aberrations are observed in about 50 % of MDS cases. The most common aberrations are 5q-, 7/7q-, trisomy 8, del(20q), and inv(3) or t(3;3).
Chromosomal translocations involving the EVI1 locus are a recurrent finding in myeloid leukemia and are associated with poor prognosis. Two common recurrent rearrangements affect the 3q26 locus. One is the inv(3)(q21q26) and the translocation t(3;3)(q21;q26), in which EVI1 overexpression is caused by juxtaposition of the EVI1 gene to enhancer elements of the Ribophorin gene at 3q21. EVI1 activation is also observed in the translocations t(3;12)(q26;p13) and t(3;21)(q26;q22) and is due to generation of the fusion genes ETV6/EVI1 and RUNX1/EVI1, respectively.
XL MECOM 3q26 hybridized to lymphocytes. One normal metaphase and one normal interphase are shown.
Normal Cell:
Two green-orange colocalization/fusion signals (2GO).
Aberrant Cell (typical results):
One green-orange colocalization/fusion signal (1GO), one separate green (1G) and orange (1O) signal each resulting from a chromosome break in the relevant locus.
Aberrant Cell (typical results):
Two green-orange colocalization/fusion signals (2GO) and one separate green (1G) signal indicating a chromosome break at a more distal region of the locus covered by the green probe.
The meeting of the German Society of Human Genetics (gfh) was held in Weimar, Germany this week and of course the attendees had the opportunity to visit the joined booth of MetaSystems and MetaSystems Probes. Main topics of the exhibition have been the latest advancements of the Neon case and imaging platform and RapidHyb, the revolutionary fast hybridization times of MetaSystems Probes' FISH probes.
Fluorescence in situ hybridization has become an essential detection assay in today´s routine diagnostics. However, long hybridization times of many hours to overnight are still a restrictive factor. We have refined the production process of our FISH probes to reduce background and artefacts and to improve the signal to noise ratio, particularly in short-time hybridization. Since mid-2015, one hour hybridization on lymphocytes is an integral part of quality control for all XCyting locus-specific probes at our manufacturing facility.
MetaSystems proudly announces the opening of our first subsidiary in the Southern hemisphere. The new office is located in Buenos Aires, Argentina and bears the name MetaSystems Latinoamérica SAS (MetaSystems LatAm). The foundation has been the response to the growing demand on technical knowledge and 'know how' experienced in the last years all over the Latin American territories. MetaSystems LatAm takes care for the organization of sales, support, and customer relation activities in all countries in Latin America. It will manage distributor companies and contacts in the region, and it will facilitate communication between end-users, distributors and headquarters in Germany.
Please select your country of residence. Choose International if your country is not listed.
Our internet site may contain information that is not approved in all countries or regions. To ensure accuracy of content, it is required that you select the site which is appropriate for your country of residence.
