XL MLL plus

Break Apart Probe

Order Number
D-5060-100-OG
Package Size
100 µl (10 Tests)
Labels
  
Chromosome
11

Description

XL MLL plus

The XL MLL plus probe is designed as a break apart probe. Its orange labeled part hybridizes proximal to the KMT2A (formerly MLL) gene locus at 11q23, the green labeled probe hybridizes to the distal region. The probe does not cover coding regions within the KMT2A gene.

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Clinical Details

A number of recurrent chromosomal abnormalities have been shown to have prognostic significance in acute lymphoblastic leukemia, especially in B-precursor ALL. Some chromosomal abnormalities, such as high hyperdiploidy and the TEL-AML1 fusion, are associated with more favorable outcomes, while others, including the t(9;22), rearrangements of the KMT2A gene (chromosome 11q23), and intrachromosomal amplification of the AML1 gene (iAMP21), are associated with a poorer prognosis.

Chromosomal rearrangements involving the human KMT2A gene are recurrently associated with the disease phenotype of acute leukemias. The identification of KMT2A gene rearrangements is necessary for rapid clinical decisions resulting in specific therapy regimens. Amplification of KMT2A in MDS and AML has also been observed, and transcriptional similarities between KMT2A amplified and KMT2A rearranged leukemias were identified.

Clinical Applications

  • Acute Lymphoblastic Leukemia (ALL)
  • Acute Myelogenous Leukemia (AML)
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Images

XL MLL plus

XL MLL plus hybridized to bone marrow cells. Two aberrant interphase nuclei with one fusion signal and one separate orange and green signal each are shown, representing a translocation with KTM2A involvement.

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Expected Patterns

Expected Pattern 1

Normal Cell:
Two green-orange (2GO) fusion signals representing the two normal KMT2A loci.

Expected Pattern 2

Aberrant Cell (typical results):
One green (1G), one orange (1O), and one green-orange (1GO) fusion signal, indicating a chromosome break in the KMT2A locus.

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Literature

  • Poppe et al (2004) Blood 103:229-235
  • Meyer et al (2006) Leukemia 20:777-784
  • Cavazzini et al (2006) Haematologica 91:381-385

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