Fluorescence in situ hybridization has become an essential detection assay in today´s routine diagnostics. However, long hybridization times of many hours to overnight are still a restrictive factor. We have refined the production process of our FISH probes to reduce background and artefacts and to improve the signal to noise ratio, particularly in short-time hybridization. Since mid-2015, one hour hybridization on lymphocytes is an integral part of quality control for all XCyting locus-specific probes at our manufacturing facility.
- Order Number
- Package Size
- 100 µl
The myelodysplastic syndromes and myeloproliferative disorders are associated with deregulated production of myeloid cells. According to WHO classification (2008) cytogenetic aberrations are observed in about 50 % of MDS cases. The most common aberrations are 5q-, 7/7q-, trisomy 8, del(20q), and inv(3) or t(3;3).
The 5q- syndrome is defined as a primary myelodysplastic syndrome (MDS) with del(5q) as the sole karyotypic abnormality and without excess of blasts. A commonly deleted region of the 5q- syndrome is approximately 1.5-megabases at 5q31-q32 and contains the EGR1 gene.
The del(5q) in AML, particularly secondary AML, invariably occurs together with other karyotypic abnormalities.
- Myelodysplastic Syndrome (MDS)
- Acute Myelogenous Leukemia (AML)
Two green (2G) and two orange (2O) signals.
Aberrant Cell (typical results):
Two green (2G) and one orange (1O) signal, indicating the deletion of the EGR1 at 5q31.
- Horrigan et al (2000) Blood 95:2372-2377
- Wei et al (2009) Proc Natl Acad Sci USA 106(31):12974-12979
- Boultwood et al (2010) Blood 16:5803-5811