Fluorescence in situ hybridization has become an essential detection assay in today´s routine diagnostics. However, long hybridization times of many hours to overnight are still a restrictive factor. We have refined the production process of our FISH probes to reduce background and artefacts and to improve the signal to noise ratio, particularly in short-time hybridization. Since mid-2015, one hour hybridization on lymphocytes is an integral part of quality control for all XCyting locus-specific probes at our manufacturing facility.
- Order Number
- Package Size
- 100 µl
The XL DLEU/TP53 locus-specific probe detects deletions in the long arm of chromosome 13 and in the short arm of chromosome 17. The orange labeled probe hybridizes to the DLEU locus region at 13q14.2, including D13S319. The green labeled probe hybridizes specifically to the TP53 gene region at 17p13.
The prognosis and clinical course of CLL are heterogeneous. Conventional banding techniques in CLL are hampered by the low mitotic index of the neoplastic cells. The introduction of interphase cytogenetics using fluorescent in situ hybridization (FISH) has greatly increased the sensitivity of cytogenetic analyses. With FISH abnormalities can be detected in more than 80 % of patients by using a 4-probe panel for the detection of trisomy 12q13-15 and deletions 13q14, 17p13, and 11q22-23. An additional 10 % of patients can be shown to carry a 6q21 deletion, 14q32 translocation, and partial trisomy 3q or 8q.
TP53 is a tumor suppressor gene that stops cell division when DNA damage is present. Loss of TP53 at 17p13 is a powerful predictor of resistance to therapy with purine analogues and alkylating agents and poor prognosis in CLL. The most frequently deleted region in B-CLL is located in 13q14.3 distal to RB1. The minimal deleted region (MDR) is 350 kb in size and includes the gene loci for the mi-RNAs 15 and 16. CLL with 13q deletion as the sole cytogenetic abnormality (del13q-only) usually have a good prognosis.
- Multiple Myeloma and Plasma Cell Neoplasms (MM)
- Chronic Lymphocytic Leukemia (CLL)
Two green (2G) and two orange (2O) signals.
Aberrant Cell (typical results):
Two green (2G) and one orange (1O) signal, indicating a deletion in 13q14.
Aberrant Cell (typical results): One green (1G) and two orange (2O) signals, indicating a deletion in17p13.
- Doehner et al (2000) N Engl J Med 34:1910-1916
- Stilgenbauer et al (2002) Leukemia 16:993-1007
- Gunn at al (2008) J Mol Diagn 10:442-451