XL DLEU/TP53

Deletion Probe

Order Number
D-5067-100-OG
Package Size
100 µl
Labels
  
Chromosomes
1317

Description

XL DLEU/TP53

The XL DLEU/TP53 locus-specific probe detects deletions in the long arm of chromosome 13 and in the short arm of chromosome 17. The orange labeled probe hybridizes to the DLEU locus region at 13q14.2, including D13S319. The green labeled probe hybridizes specifically to the TP53 gene region at 17p13.

Clinical Details

The prognosis and clinical course of CLL are heterogeneous. Conventional banding techniques in CLL are hampered by the low mitotic index of the neoplastic cells. The introduction of interphase cytogenetics using fluorescent in situ hybridization (FISH) has greatly increased the sensitivity of cytogenetic analyses. With FISH abnormalities can be detected in more than 80 % of patients by using a 4-probe panel for the detection of trisomy 12q13-15 and deletions 13q14, 17p13, and 11q22-23. An additional 10 % of patients can be shown to carry a 6q21 deletion, 14q32 translocation, and partial trisomy 3q or 8q.

TP53 is a tumor suppressor gene that stops cell division when DNA damage is present. Loss of TP53 at 17p13 is a powerful predictor of resistance to therapy with purine analogues and alkylating agents and poor prognosis in CLL. The most frequently deleted region in B-CLL is located in 13q14.3 distal to RB1. The minimal deleted region (MDR) is 350 kb in size and includes the gene loci for the mi-RNAs 15 and 16. CLL with 13q deletion as the sole cytogenetic abnormality (del13q-only) usually have a good prognosis.

Clinical Applications

  • Multiple Myeloma and Plasma Cell Neoplasms (MM)
  • Chronic Lymphocytic Leukemia (CLL)

Images

XL DLEU/TP53

XL DLEU/TP53 hybridized to bone marrow cells. A cell with normal signal pattern together with cells with a deletion of TP53 (2 orange, 1 green) and cells with deletions of TP53 and DLEU (1 orange, 1 green) are shown.

Expected Patterns

Expected Pattern 1

Normal Cell:
Two green (2G) and two orange (2O) signals.

Expected Pattern 2

Aberrant Cell (typical results):
Two green (2G) and one orange (1O) signal, indicating a deletion in 13q14.

Expected Pattern 3

Aberrant Cell (typical results): One green (1G) and two orange (2O) signals, indicating a deletion in17p13.

Literature

  • Doehner et al (2000) N Engl J Med 34:1910-1916
  • Stilgenbauer et al (2002) Leukemia 16:993-1007
  • Gunn at al (2008) J Mol Diagn 10:442-451

Downloads

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