XL RB1/DLEU/LAMP

Deletion Probe

Order Number
D-5070-100-TC
Package Size
100 µl
Labels
   
Chromosome
13

Description

XL RB1/DLEU/LAMP

The XL RB1/DLEU/LAMP locus-specific probe can detect variants of deletions in the long arm of chromosome 13. The orange labeled probe hybridizes to the DLEU locus region at 13q14.2, including D13S319. A green labeled probe hybridizes specifically to the more proximal RB1 gene region at 13q14.2. A blue labeled probe is added as control which hybridizes to the LAMP gene locus at 13q34.

Clinical Details

In chronic lymphocytic leukemia (CLL), deletion at 13q14 with at least one allele occurs in more than 50 % of cases. A minimal deleted region (MDR) of 350 kb in size has been identified and is usually seen as an indicator for good prognosis. However, recent studies using high resolution arrays have shown, that the 13q14 deletion can vary in size and if including the RB1 gene may lead to a change in the prognosis of CLL patients. RB1 is a critical regulator of cell cycle progression and genomic stability, and its loss could explain this change of interpretation in CLL.


Deletions of chromosome 13q14 have been reported in a variety of human tumors, including other types of lymphoid tumors and myeloid leukemias, as well as prostate, head and neck, and non-small-cell lung cancers.

Clinical Applications

  • Chronic Lymphocytic Leukemia (CLL)
  • Multiple Myeloma and Plasma Cell Neoplasms (MM)

Images

XL RB1/DLEU/LAMP

XL RB1/DLEU/LAMP hybridized to lymphocytes. Two normal interphases are shown.

Expected Patterns

Expected Pattern 1

Normal Cell:
Two green (2G), two orange (2O), and two blue (2B) signals.

Expected Pattern 2

Aberrant Cell:
Two green (2G), one orange (1O), and two blue (2B) signals.

Expected Pattern 3

Aberrant Cell: One green (1G), one orange (1O), and two blue (2B) signals.

Expected Pattern 4

Aberrant Cell: One green (1G), one orange (1O), and one blue (1B) signal.

Literature

  • Doehner et al (2000) N Engl J Med 34:1910-1916
  • Ouillette et al (2008) Cancer Res 68:1012-1021
  • Dal Bo et al (2011) Genes Chromosomes Cancer 50:633-643

Downloads

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