Fluorescence in situ hybridization has become an essential detection assay in today´s routine diagnostics. However, long hybridization times of many hours to overnight are still a restrictive factor. We have refined the production process of our FISH probes to reduce background and artefacts and to improve the signal to noise ratio, particularly in short-time hybridization. Since mid-2015, one hour hybridization on lymphocytes is an integral part of quality control for all XCyting locus-specific probes at our manufacturing facility.
- Order Number
- Package Size
- 100 µl
The XL RB1/DLEU/LAMP locus-specific probe can detect variants of deletions in the long arm of chromosome 13. The orange labeled probe hybridizes to the DLEU locus region at 13q14.2, including D13S319. A green labeled probe hybridizes specifically to the more proximal RB1 gene region at 13q14.2. A blue labeled probe is added as control which hybridizes to the LAMP gene locus at 13q34.
In chronic lymphocytic leukemia (CLL), deletion at 13q14 with at least one allele occurs in more than 50 % of cases. A minimal deleted region (MDR) of 350 kb in size has been identified and is usually seen as an indicator for good prognosis. However, recent studies using high resolution arrays have shown, that the 13q14 deletion can vary in size and if including the RB1 gene may lead to a change in the prognosis of CLL patients. RB1 is a critical regulator of cell cycle progression and genomic stability, and its loss could explain this change of interpretation in CLL.
Deletions of chromosome 13q14 have been reported in a variety of human tumors, including other types of lymphoid tumors and myeloid leukemias, as well as prostate, head and neck, and non-small-cell lung cancers.
- Chronic Lymphocytic Leukemia (CLL)
- Multiple Myeloma and Plasma Cell Neoplasms (MM)
Two green (2G), two orange (2O), and two blue (2B) signals.
Two green (2G), one orange (1O), and two blue (2B) signals.
Aberrant Cell: One green (1G), one orange (1O), and two blue (2B) signals.
Aberrant Cell: One green (1G), one orange (1O), and one blue (1B) signal.
- Doehner et al (2000) N Engl J Med 34:1910-1916
- Ouillette et al (2008) Cancer Res 68:1012-1021
- Dal Bo et al (2011) Genes Chromosomes Cancer 50:633-643