Fluorescence in situ hybridization has become an essential detection assay in today´s routine diagnostics. However, long hybridization times of many hours to overnight are still a restrictive factor. We have refined the production process of our FISH probes to reduce background and artefacts and to improve the signal to noise ratio, particularly in short-time hybridization. Since mid-2015, one hour hybridization on lymphocytes is an integral part of quality control for all XCyting locus-specific probes at our manufacturing facility.
Break Apart Probe
- Order Number
- Package Size
- 100 µl
Several recurrent balanced translocations and inversions, and their variants, are
recognized in the WHO category AML with recurrent genetic abnormalities. Furthermore, several cytogenetic abnormalities are considered sufficient to establish the WHO diagnosis of AML with myelodysplasia-related features when 20% or more blood or marrow blasts are present.
Translocations involving nucleoporin 98kD (NUP98) on chromosome 11p15 occur at relatively low frequency in acute myeloid leukemia (AML), but can be missed with routine karyotyping. NUP98 is known to be fused to at least 28 different partner genes
in patients with hematopoietic malignancies, including acute myeloid leukemia, chronic
myeloid leukemia in blast crisis, myelodysplastic syndrome, acute lymphoblastic
leukemia, and bilineage/biphenotypic leukemia.
- Acute Myelogenous Leukemia (AML)
- Acute Lymphoblastic Leukemia (ALL)
- Myelodysplastic Syndrome (MDS)
Two green-orange fusion signals (2GO).
Aberrant Cell (typical results):
One green (1G), one orange (1O), and one green-orange (1GO) fusion signal, indicating a chromosome break in the NUP98 locus.
- Nebral et al (2005) Haematologica 90: 746- 752
- Romana et al (2006) Leukemia 20: 696-706
- Gough et al (2011) Blood 118: 6247-6257