XL NUP98

Break Apart Probe

Order Number
D-5077-100-OG
Package Size
100 µl
Labels
  
Chromosome
11

Description

XL NUP98

The XL NUP98 probe is designed as a break apart probe. Its orange labeled part hybridizes proximal to the NUP98 gene at 11p15, the green labeled probe hybridizes to the distal region of NUP98.

Clinical Details

Several recurrent balanced translocations and inversions, and their variants, are
recognized in the WHO category AML with recurrent genetic abnormalities. Furthermore, several cytogenetic abnormalities are considered sufficient to establish the WHO diagnosis of AML with myelodysplasia-related features when 20% or more blood or marrow blasts are present.

Translocations involving nucleoporin 98kD (NUP98) on chromosome 11p15 occur at relatively low frequency in acute myeloid leukemia (AML), but can be missed with routine karyotyping. NUP98 is known to be fused to at least 28 different partner genes
in patients with hematopoietic malignancies, including acute myeloid leukemia, chronic
myeloid leukemia in blast crisis, myelodysplastic syndrome, acute lymphoblastic
leukemia, and bilineage/biphenotypic leukemia.

Clinical Applications

  • Acute Myelogenous Leukemia (AML)

Images

XL NUP98

XL NUP98 was hybridized to lymphocytes. One normal interphase is shown.

Expected Patterns

Expected Pattern 1

Normal Cell:
Two green-orange fusion signals (2GO).

Expected Pattern 2

Aberrant Cell (typical results):
One green (1G), one orange (1O), and one green-orange (1GO) fusion signal, indicating a chromosome break in the NUP98 locus.

Literature

  • Nebral et al (2005) Haematologica 90: 746- 752
  • Romana et al (2006) Leukemia 20: 696-706
  • Gough et al (2011) Blood 118: 6247-6257

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