XL 6q21/6q23/6cen

Deletion Probe

Order Number
D-5088-100-TC
Package Size
100 µl (10 Tests)
Labels
   
Chromosome
6

Description

XL 6q21/6q23/6cen

XL 6q21/6q23/6cen consists of a green-labeled probe hybridizing to the SEC63 gene region at 6q21, an orange-labeled probe hybridizing to the MYB gene region at 6q23.3 and an aqua-labeled probe hybridizing to the centromere of chromosome 6.

Probe maps are created in accordance with the intended purpose of the product. Solid colored bars do not necessarily indicate that the probe fully covers the indicated genomic region. Therefore, caution is advised when interpreting results generated through off-label use. Probe map details based on UCSC Genome Browser GRCh37/hg19. Map components not to scale. Further information is available on request.

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Clinical Details

The prognosis and clinical course of chronic lymphocytic leukemia (CLL) is heterogeneous. Conventional banding techniques in CLL are hampered by the low mitotic index of the neoplastic cells. The introduction of interphase cytogenetics using fluorescent in situ hybridization (FISH) has greatly increased the sensitivity of cytogenetic analyses. With FISH, abnormalities can be detected in more than 80% of patients by using a 4-probe panel for the detection of trisomy 12q13-15 and deletions 13q14, 17p13, and 11q22-23. An additional 10% of patients can be shown to carry a 6q21 deletion, 14q32 translocation, and partial trisomy 3q or 8q.
Deletions involving the long arm of chromosome 6 (6q) are among the most common structural aberrations leading to a loss of chromosomal material in lymphoproliferative disorders and non-Hodgkin lymphoma (NHL). Two distinct regions, one at 6q21-22.1, the other at 6q23.3-25, have been found as minimal deleted regions in 6q- patients. A 6q deletion has been found in a variety of other human malignancies as well, including breast carcinoma, malignant melanoma, renal cell carcinoma, salivary gland adenocarcinoma, ovarian carcinoma, acute lymphoblastic leukemia, and nodal NHL.

Clinical Applications

  • Acute Lymphoblastic Leukemia (ALL)
  • Chronic Lymphocytic Leukemia (CLL)
  • Non-Hodgkin Lymphomas (NHL)
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Images

XL 6q21/6q23/6cen

XL 6q21/6q23/6cen hybridized to lymphocytes. Two normal interphases are shown.

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Expected Patterns

Expected Pattern 1

Normal Cell:
Two green (2G), two orange (2O), and two blue (2B) signals.

Expected Pattern 2

Aberrant Cell (typical results):
One green (1G), one orange (1O), and two blue (2B) signals indicating the deletion of a 6q23 locus and of a 6q21 locus.

Expected Pattern 3

Aberrant Cell (typical results):
Two green (2G), one orange (1O), and two blue (2B) signals indicating the deletion of a 6q23 locus or one green (1G), two orange (2O), and 2 blue (2B) signals, indicating the deletion of a 6q21 locus.

Expected Pattern 4

Aberrant Cell (typical results):
One green (1G), one orange (1O), and one blue (1B) signal indicating a monosomy 6.

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Literature

  • Stilgenbauer et al (1999) Leukemia 13:1331-1334
  • Zhang et al (2000) Genes Chrom Canc 27:52-58
  • Starostik et al (2000) Blood 95:1180-1187

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