XL TP53/NF1

Deletion Probe

Order Number
D-5089-100-OG
Package Size
100 µl
Labels
  
Chromosome
17

Description

XL TP53/NF1

The XL TP53/NF1 probe detects deletions which occur in 17p13 and involve the TP53 gene locus. The orange labeled probe hybridizes to a larger TP53 specific region while a green labeled probe which hybridizes to the NF1 gene region at 17q11.2 functions as a reference probe.

Clinical Details

TP53 is described as 'the guardian of the genome' because of its role in conserving stability by preventing genome mutation. Therefore TP53 is classified as a tumor suppressor gene. It has many mechanisms of anticancer function, and plays a role in apoptosis, genomic stability, and inhibition of angiogenesis. If the TP53 gene is damaged tumor suppression is severely reduced.

The TP53 gene is located on the short arm of chromosome 17 (17p13.1). More than 50 % of human tumors contain either a mutation or deletion of the TP53 gene.

Clinical Applications

  • Chronic Lymphocytic Leukemia (CLL)
  • Multiple Myeloma and Plasma Cell Neoplasms (MM)
  • Chronic Myelogenous Leukemia (CML)

Images

XL TP53/NF1

XL TP53/NF1 hybridized to lymphocytes. One normal metaphase is shown.

Expected Patterns

Expected Pattern 1

Normal Cell: Two green (2G) and two orange (2O) signals.

Expected Pattern 2

Aberrant Cell (typical results):
Two green (2G) and one orange (1O) signal, indicating a deletion of theTP53 locus.

Expected Pattern 3

Aberrant Cell (typical results):
One green (1G) and one orange (1O) signal indicating the deletion of the TP53 and NF1 locus.

Expected Pattern 4

Aberrant Cell (typical results): Three green (3G) and one orange (1O) signal, indicating the presence of isochromosome 17q.

Literature

  • Fioretos et al (1999) Blood 94:225-232
  • Barbouti et al (2004) Am J Hum Genet 74:1-10
  • Carvalho et al (2008) Genome Res 18:1724-1732

Downloads

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