XL ATM/11cen

Deletion Probe

Order Number
D-5102-100-OG
Package Size
100 µl
Labels
  
Chromosome
11

Description

XL ATM/11cen

The XL ATM/11cen locus-specific probe detects deletions in 11q22.3. This probe is labeled in orange and spans from marker D11S2179 within the ATM gene to the more proximal located NPAT region. A green labeled probe, specific for the centromere of chromosome 11, functions as a reference probe.

Please note that this probe is the replacement for XL ATM (D-5011-100-OG).

Clinical Details

The prognosis and clinical course of CLL are heterogeneous. Conventional banding techniques in CLL are hampered by the low mitotic index of the neoplastic cells. The introduction of interphase cytogenetics using fluorescent in situ hybridization (FISH) has greatly increased the sensitivity of cytogenetic analyses. With FISH abnormalities can be detected in more than 80% of patients by using a 4-probe panel for the detection of trisomy 12q13-15 and deletions 13q14, 17p13, and 11q22-23. An additional 10 % of patients can be shown to carry a 6q21 deletion, 14q32 translocation, and partial trisomy 3q or 8q.

Chromosome 11q22.3-23.1 deletions involving the ataxia-telangiectasia mutated (ATM) locus are detected at diagnosis in 15 - 20 % of cases of B-cell chronic lymphocytic leukemia (CLL) and are associated with a more aggressive disease.

Clinical Applications

  • Chronic Lymphocytic Leukemia (CLL)

Images

XL ATM/11cen

XL ATM/11cen hybridized to lymphocytes. One normal interphase and metaphase are shown.

Expected Patterns

Expected Pattern 1

Normal Cell:
Two green (2G) and two orange (2O) signals.

Expected Pattern 2

Aberrant Cell (typical results):
Two green (2G) and one orange (1O) signal indicating the deletion of ATM at 11q22.

Literature

  • Doehner et al (1997) Blood 89:2516-2522
  • Cuneo et al (2002) Haematologica 87:44-51
  • Tsimberidou et al (2009) Cancer 115:373-380

Downloads

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