XL 5q32 PDGFRB BA

Break Apart Probe

Order Number
D-5104-100-OG
Package Size
100 µl
Labels
  
Chromosome
5

Description

XL 5q32 PDGFRB BA

The XL 5q32 PDGFRB BA probe is designed as a break apart probe. The orange labeled probe hybridizes proximal to the breakpoint in the PDGFRB gene region at 5q32, the green labeled probe hybridizes distal to the breakpoint.

Clinical Details

The updated (2016) World Health Organization (WHO) classification of tumors of the hematopoietic and lymphoid tissues indicates the category myeloid/lymphoid neoplasms with eosinophilia and rearrangement of PDGFRA, PDGFRB, FGFR1, or with PCM1-JAK2.

Myeloid neoplasms (MPNs) with rearrangement of PDGFRB are phenotypically and genotypically diverse. The fusion genes involving PDGFRB described to date have been associated with cytogenetically detectable translocations. MPNs associated with rearrangement of PDGFRB are responsive to Imatinib.

Clinical Applications

  • Chronic Myelogenous Leukemia and Myeloproliferative Neoplasms (CML/MPN)

Images

XL 5q32 PDGFRB BA

XL 5q32 PDGFRB BA hybridized to lymphocytes. Three normal interphases are shown.

Expected Patterns

Expected Pattern 1

Normal Cell:
Two green-orange colocalization/fusion signals (2GO).

Expected Pattern 2

Aberrant Cell (typical results):
One green-orange colocalization/fusion signal (1GO), one separate green (1G) and orange (1O) signal each resulting from a chromosome break in the relevant locus.

Literature

  • Wlodarska et al (1997) Blood 89:1716-1722
  • Apperley et al (2002) N Engl J Med 347:481-487
  • Wilkinson et al (2003) Blood 102:4187-4190

Downloads

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