Fluorescence in situ hybridization has become an essential detection assay in today´s routine diagnostics. However, long hybridization times of many hours to overnight are still a restrictive factor. We have refined the production process of our FISH probes to reduce background and artefacts and to improve the signal to noise ratio, particularly in short-time hybridization. Since mid-2015, one hour hybridization on lymphocytes is an integral part of quality control for all XCyting locus-specific probes at our manufacturing facility.
XL t(14;20) IGH/MAFB DF
Translocation/Dual Fusion Probe
- Order Number
- Package Size
- 100 µl
The most frequent primary abnormalities in multiple myeloma (MM) are trisomies of odd-numbered chromosomes or translocations involving the immunglobulin heavy chain (IgH) gene locus. The most common MM-associated IGH translocations are t(11;14), t(4;14), t(6;14), t(14;16) and t(14;20) in the order of their occurrence. The consequence of these rearrangements is the dysregulation of genes juxtaposed to transcriptional enhancers in the IGH locus. Prognosis and risk stratification strongly depends on the detection and interpretation of cytogenetic primary abnormalities. t(14;16) and t(14;20) are considered as high risk, t(4;14) as intermediate risk and t(6;14) and t(11;14) as standard risk cytogenetic aberrations in patients with MM based on FISH testing. Secondary aberrations are also influencing the outcome.
Even if associated with poor prognosis in MM, MGUS/SMM cases characterized by t(14;20) can be stable for years before progression occurs whereas MGUS/SMM cases with t(4;14) and t(14;16) showing a higher progression rate. The recurrent t(14;20) results in ectopic expression of the basic leucine zipper transcription factor MAFB which has an important role in lineage-specific hematopoiesis.
- Multiple Myeloma and Plasma Cell Neoplasms (MM)
Two green (2G) and two orange (2O) signals.
Aberrant Cell (typical results):
One green (1G), one orange (1O), and two green-orange colocalization/fusion signals (2GO) resulting from a reciprocal translocation between the relevant loci.
- Boersma-Vreugdenhil et al (2004) Brit J Haem 126:355-363
- Ross et al (2010) Haematologica 95:1221-1225
- Rajan and Rajkumar (2015) Blood Cancer J. 5:e365