XL TCRA/D

Break Apart Probe

Order Number
D-5106-100-OG
Package Size
100 µl (10 Tests)
Labels
  
Chromosome
14

Description

XL TCRA/D

XL TCRA/D consists of an orange-labeled probe hybridizing proximal to the TCRA/D gene region at 14q11.2 and a green-labeled probe hybridizing distal to TCRA/D gene region at 14q11.2.

Probe maps are created in accordance with the intended purpose of the product. Solid colored bars do not necessarily indicate that the probe fully covers the indicated genomic region. Therefore, caution is advised when interpreting results generated through off-label use. Probe map details based on UCSC Genome Browser GRCh37/hg19. Map components not to scale. Further information is available on request.

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Clinical Details

Chromosomal aberrations with breakpoints in T-cell receptor (TCR) gene loci are recurrent in several T-cell malignancies. The chromosomal alterations juxtapose oncogenes next to TCR regulatory sequences leading to deregulated expression of those oncogenes.

T-cell prolymphocytic leukemia (T-PLL) harbors frequent alterations of the TCRA/D locus, usually caused by an inv(14)(q11q32). By molecular cytogenetic studies, the incidence of TCRA/D rearrangements is about 24% of all T-cell acute lymphoblastic leukemia (T-ALL) cases.

Clinical Applications

  • Acute Lymphoblastic Leukemia (ALL)
  • Non-Hodgkin Lymphomas (NHL)
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Images

XL TCRA/D

XL TCRA/D hybridized to lymphocytes. One normal metaphase is shown.

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Expected Patterns

Expected Pattern 1

Normal Cell:
Two green-orange fusion signals (2GO).

Expected Pattern 2

Aberrant Cell (typical results):
One green (1G), one orange (1O), and one green-orange (1GO) fusion signal, indicating a chromosome break in the TCRA/D locus.

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Literature

  • Gesk et al (2003) Leukemia 17:738-745
  • Leich et al (2007) J Pathol 213:99-105
  • Feldman et al (2009) Am J Clin Pathol 130:178-185

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