Fluorescence in situ hybridization has become an essential detection assay in today´s routine diagnostics. However, long hybridization times of many hours to overnight are still a restrictive factor. We have refined the production process of our FISH probes to reduce background and artefacts and to improve the signal to noise ratio, particularly in short-time hybridization. Since mid-2015, one hour hybridization on lymphocytes is an integral part of quality control for all XCyting locus-specific probes at our manufacturing facility.
Break Apart Probe
- Order Number
- Package Size
- 100 µl
Chromosomal aberrations with breakpoints in T-cell receptor (TCR) gene loci are recurrent in several T-cell malignancies. The chromosomal alterations juxtapose oncogenes next to TCR regulatory sequences leading to deregulated expression of those oncogenes.
T-cell prolymphocytic leukemia (T-PLL) harbors frequent alterations of the TCRA/D locus, usually caused by an inv(14)(q11q32). By molecular cytogenetics studies, the incidence of TCRA/D rearrangements is about 24% of all T-ALL cases.
- Acute Lymphoblastic Leukemia (ALL)
- Non-Hodgkin Lymphomas (NHL)
Two green-orange fusion signals (2GO).
Aberrant Cell (typical results):
One green (1G), one orange (1O), and one green-orange (1GO) fusion signal, indicating a chromosome break in the TCRA/D locus.
- Gesk et al (2003) Leukemia 17: 738-745
- Leich et al (2007) J Pathol 213: 99-105
- Feldman et al (2009) Am J Clin Pathol 130: 178-185