The meeting of the German Society of Human Genetics (gfh) was held in Weimar, Germany this week and of course the attendees had the opportunity to visit the joined booth of MetaSystems and MetaSystems Probes. Main topics of the exhibition have been the latest advancements of the Neon case and imaging platform and RapidHyb, the revolutionary fast hybridization times of MetaSystems Probes' FISH probes.
XL IGH BA
Break Apart Probe
- Order Number
- Package Size
- 100 µl (10 Tests)
Chromosomal translocations affecting the IGH locus at 14q32.3 are recurrent in many types of lymphomas and plasma cell neoplasms. The consequence of these rearrangements is the dysregulation of genes juxtaposed to transcriptional enhancers in the IGH locus. The Burkitt lymphoma is a rare but fast growing type of NHL. The translocation between the MYC gene locus at 8q24 and the immunoglobulin genes (IG) for the kappa light chain at 2p12 (IGK), for the heavy chain at 14q32 (IGH) or for the lambda light chain at 22q11 (IGL) juxtapose the MYC gene to an IG enhancer. About 80% of Burkitt lymphoma patients have the MYC rearrangement t(8;14) while approximately 10% show a translocation between the MYC gene region and IGK or IGL. The Follicular lymphoma (FL) is the most common indolent form of the Non-Hodgkin lymphomas (NHL). The reciprocal translocation t(14;18) is observed in about 85% of patients with FL and results in overexpression of the BCL-2 protein which is involved in the regulation of apoptosis. Around 1% of all cancers and 10% of hematologic malignancies are caused by Multiple Myelomas (MM). Translocations affecting the IGH locus are observed in about 40% of MM cases.
Due to the telomeric position of the IgH locus, 14q32.3 translocations may be easily missed by conventional cytogenetics. FISH diagnostic is therefore a valuable tool in the diagnostic of translocations affecting the IGH locus.
- Multiple Myeloma and Plasma Cell Neoplasms (MM)
- Non-Hodgkin Lymphomas (NHL)
- Acute Lymphoblastic Leukemia (ALL)
Two green-orange colocalization/fusion signals (2GO).
Aberrant Cell (typical results):
One green-orange colocalization/fusion signal (1GO), one separate green (1G) and orange (1O) signal each resulting from a chromosome break in the relevant locus.
- Freedman (2014) Am J Hematol 89: 429-436
- Rajan and Rajkumar (2015) Blood Canc J 5:1-7
- Nguyen et al (2017) Genes 8:1-23