Fluorescence in situ hybridization has become an essential detection assay in today´s routine diagnostics. However, long hybridization times of many hours to overnight are still a restrictive factor. We have refined the production process of our FISH probes to reduce background and artefacts and to improve the signal to noise ratio, particularly in short-time hybridization. Since mid-2015, one hour hybridization on lymphocytes is an integral part of quality control for all XCyting locus-specific probes at our manufacturing facility.
XL IGH BA
Break Apart Probe
- Order Number
- Package Size
- 100 µl
Chromosomal translocations affecting the IGH locus at 14q32.3 are recurrent in many types of lymphomas and plasma cell neoplasms. The consequence of these rearrangements is the dysregulation of genes juxtaposed to transcriptional enhancers in the IGH locus. The Burkitt lymphoma is a rare but fast growing type of NHL. The translocation between the MYC gene locus at 8q24 and the immunoglobulin genes (IG) for the kappa light chain at 2p12 (IGK), for the heavy chain at 14q32 (IGH) or for the lambda light chain at 22q11 (IGL) juxtapose the MYC gene to an IG enhancer. About 80% of Burkitt lymphoma patients have the MYC rearrangement t(8;14) while approximately 10% show a translocation between the MYC gene region and IGK or IGL. The Follicular lymphoma (FL) is the most common indolent form of the Non-Hodgkin lymphomas (NHL). The reciprocal translocation t(14;18) is observed in about 85% of patients with FL and results in overexpression of the BCL-2 protein which is involved in the regulation of apoptosis. Around 1% of all cancers and 10% of hematologic malignancies are caused by Multiple Myelomas (MM). Translocations affecting the IGH locus are observed in about 40% of MM cases.
Due to the telomeric position of the IgH locus, 14q32.3 translocations may be easily missed by conventional cytogenetics. FISH diagnostic is therefore a valuable tool in the diagnostic of translocations affecting the IGH locus.
- Multiple Myeloma and Plasma Cell Neoplasms (MM)
- Non-Hodgkin Lymphomas (NHL)
- Acute Lymphoblastic Leukemia (ALL)
Two green-orange colocalization/fusion signals (2GO).
Aberrant Cell (typical results):
One green-orange colocalization/fusion signal (1GO), one separate green (1G) and orange (1O) signal each resulting from a chromosome break in the relevant locus.
- Freedman (2014) Am J Hematol 89: 429-436
- Rajan and Rajkumar (2015) Blood Canc J 5:1-7
- Nguyen et al (2017) Genes 8:1-23