XL t(4;14) FGFR3/IGH DF

Translocation/Dual Fusion Probe

Order Number
D-5108-100-OG
Package Size
100 µl
Labels
  
Chromosomes
414

Description

XL t(4;14) FGFR3/IGH DF

XL t(4;14) FGFR3/IGH DF is designed as a dual fusion probe. The orange labeled probe spans the breakpoint at 4p16 (FGFR3), the green labeled probe flanks the IGH breakpoint region at 14q32.

Clinical Details

The most frequent primary abnormalities in multiple myeloma (MM) are trisomies of odd-numbered chromosomes or translocations involving the immunglobulin heavy chain (IgH) gene locus. The most common MM-associated IGH translocations are t(11;14), t(4;14), t(6;14), t(14;16) and t(14;20) in the order of their occurrence. The consequence of these rearrangements is the dysregulation of genes juxtaposed to transcriptional enhancers in the IGH locus. Prognosis and risk stratification strongly depends on the detection and interpretation of cytogenetic primary abnormalities. t(14;16) and t(14;20) are considered as high risk, t(4;14) as intermediate risk and t(6;14) and t(11;14) as standard risk cytogenetic aberrations in patients with MM based on FISH testing. Secondary aberrations are also influencing the outcome.
The recurrent translocation t(4;14)(p16;q32) is juxtaposing FGFR3 with the IGH Ea enhancer on der(14) whereas expression of NSD2 is controlled by the Eµ enhancer on der(4). FGFR3 overexpression is detectable in about 70% of t(4;14) positive MM patients, suggesting that FGFR3 dysregulation is not the crucial oncogenic event. The poor outcome of patients lacking FGFR3 expression due to the loss of der(14) is supporting this conclusion. Transcripts from the NDS2 locus on the other hand are found to be overexpressed in all t(4;14) positive MM cases, suggesting that this gene region plays a major role in the manifestation of MM.

Clinical Applications

  • Multiple Myeloma and Plasma Cell Neoplasms (MM)

Images

XL t(4;14) FGFR3/IGH DF

XL t(4;14) IGH/FGFR3 DF hybridized to lymphocytes. Two normal interphases are shown.

Expected Patterns

Expected Pattern 1

Normal Cell:
Two green (2G) and two orange (2O) signals.

Expected Pattern 2

Aberrant Cell (typical results):
One green (1G), one orange (1O), and two green-orange colocalization/fusion signals (2GO) resulting from a reciprocal translocation between the relevant loci.

Literature

  • Chesi et al (1998) Blood 92:3025-3034
  • Keats et al (2005) Blood 15:4060-4069
  • Rajan and Rajkumar (2015) Blood Cancer J. 5:e365

Downloads

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