Fluorescence in situ hybridization has become an essential detection assay in today´s routine diagnostics. However, long hybridization times of many hours to overnight are still a restrictive factor. We have refined the production process of our FISH probes to reduce background and artefacts and to improve the signal to noise ratio, particularly in short-time hybridization. Since mid-2015, one hour hybridization on lymphocytes is an integral part of quality control for all XCyting locus-specific probes at our manufacturing facility.
XL t(14;18) IGH/BCL2 DF
Translocation/Dual Fusion Probe
- Order Number
- Package Size
- 100 µl
Rearrangements of the immunoglobulin heavy chain (IGH) gene locus are present in about 50% of all Non-Hodgkin lymphomas (NHL) including follicular lymphomas (FL) and diffuse large B-cell lymphomas (DLBCL). The FL is the most common indolent form of NHL and accounts for about 20%-30% of all lymphoid tumors. The reciprocal translocation t(14;18)(q32;q21) can be detected in about 85% of patients with FL and in up to 35% of patients with DLBCL. t(14;18) juxtaposes BCL2 to transcriptional enhancers in the IGH locus and results in overexpression of the BCL-2 protein in neoplastic follicles. BCL2 is involved in the regulation of programmed cell death and shows anti-apoptotic characteristics. Overexpression of BCL2 leads to a high number of B cells having a prolonged lifespan in germinal centers. This increases the chance to acquire additional chromosomal alterations required for the neoplastic transformation of B cells. Secondary mutations promoting the transformation process are among others t(14;18) and the deletion of the TP53 and CDKN2A genes.
- Non-Hodgkin Lymphomas (NHL)
Two green (2G) and two orange (2O) signals.
Aberrant Cell (typical results):
One green (1G), one orange (1O), and two green-orange colocalization/fusion signals (2GO) resulting from a reciprocal translocation between the relevant loci.
- Bernicot et al (2005) Anticancer Res 25:3179-3182
- Ott and Rosenwald (2008) Haemotologica 93:1773-1776
- Freedman (2014) Am J Hematol 89: 429-436