XL t(8;14) MYC/IGH DF 8cen

Translocation/Dual Fusion and Amplification Probe

Order Number
D-5125-100-TC
Package Size
100 µl (10 Tests)
Labels
   
Chromosomes
814

Description

XL t(8;14) MYC/IGH DF 8cen

XL t(8;14) MYC/IGH DF 8cen consists of an aqua-labeled probe hybridizing to the centromere of chromosome 8, an orange-labeled probe hybridizing to the MYC gene region at 8q24.21 and a green-labeled probe hybridizing to the IGH gene region at 14q32.3.

Probe maps are created in accordance with the intended purpose of the product. Solid colored bars do not necessarily indicate that the probe fully covers the indicated genomic region. Therefore, caution is advised when interpreting results generated through off-label use. Probe map details based on UCSC Genome Browser GRCh37/hg19. Map components not to scale. Further information is available on request.

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Clinical Details

Chromosomal translocations involving the IGH locus are recurrent in many types of lymphomas. Burkitt lymphoma (BL) is a rare, but fast growing type of non-Hodgkin lymphoma (NHL). The translocation between the MYC gene locus at 8q24 and the immunoglobulin genes (IG) for the kappa light chain at 2p12 (IGK), for the heavy chain at 14q32 (IGH) or for the lambda light chain at 22q11 (IGL), juxtapose the MYC gene to an IG enhancer, resulting in overexpression of MYC. About 80% of BL patients show the MYC rearrangement t(8;14)(q24;q32) while approximately 10% show a translocation between the MYC gene region and IGK or IGL. Additional breakpoints in BCL2, BCL6 and CCND1 are indicators for an aggressive course and short overall survival.
MYC translocations are also present in other types of lymphomas such as diffuse large B-cell lymphoma (DLBCL), which is difficult to distinguish from BL by morphology and immunophenotype alone. The use of different techniques including FISH, genomic and cytogenetic profiling can provide additional information.

Clinical Applications

  • Non-Hodgkin Lymphomas (NHL)
  • Acute Lymphoblastic Leukemia (ALL)
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Images

XL t(8;14) MYC/IGH DF 8cen

XL t(8;14) MYC/IGH DF 8cen hybridized to lymphocytes. One normal interphase and metaphase are shown.

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Expected Patterns

Expected Pattern 1

Normal Cell:
Two blue (2B), two green (2G), and two orange (2O) signals.

Expected Pattern 2

Aberrant Cell (typical results):
Two blue (2B), one green (1G), one orange (1O), and two green-orange colocalization/fusion signals (2GO) resulting from a reciprocal translocation between the relevant loci.

Expected Pattern 3

Aberrant Cell (typical results):
Three blue (3B), two green (2G), and three orange (3O) signals resulting from a trisomy of the orange/blue labeled chromosome.

Expected Pattern 4

Aberrant Cell (typical results):
Two blue (2B), two green (2G), and three orange (3O) signals resulting from a reciprocal translocation between the orange labeled and an unknown chromosome.

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Literature

  • Siebert et al (1998) Blood 91:984-990
  • Boerma et al (2009) Leukemia 23:225-234
  • Nguyen et al (2017) Genes 8:1-23

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