XL BCL3 BA

Break Apart Probe

Order Number
D-5128-100-OG
Package Size
100 µl
Labels
  
Chromosome
19

Description

XL BCL3 BA

XL BCL3 BA is designed as a break apart probe. The orange labeled probe hybridizes proximal to the breakpoint in the BCL3 gene region at 19q13.3, the green labeled probe hybridizes distal to the breakpoint and spans the PVRL2 gene.

Clinical Details

Chronic lymphocytic leukemia (CLL) is the most common leukemia in adults. The clinical course is heterogeneous and ranges from good outcome to very aggressive and fast progressing disease. CLL is not characterized by a well-defined chromosomal translocation as many other lymphoid neoplasms. The most frequent aberrations are deletions on 6q21 (3-6%), 11q22-23 (5-20%), 13q14.3 (>50%) or 17q13.1 (3-8%) and trisomy 12 (10-20%). The recurrent t(14;19)(q32.3;q13.3) is a rare event with an incidence of <0.1% in B-cell neoplasms and is often associated with trisomy 12 or a complex karyotype. It is considered as a poor prognostic marker in CLL with inferior outcome. The translocation juxtaposes BCL3 with the immunoglobulin heavy chain gene region on chromosome 14 resulting in overexpression of BCL3. BCL3 is an oncogene and is involved in the regulation of NF-kappa-B target genes. Since it is difficult to obtain metaphases from CLL patients, interphase FISH offers great advantage over conventional cytogenetics.

Clinical Applications

  • Chronic Lymphocytic Leukemia (CLL)

Images

XL BCL3 BA

XL BCL3 BA hybridized to bone marrow cells. One aberrant cell with split signal is shown, indicating a break in the BCL3 locus.

Expected Patterns

Expected Pattern 1

Normal Cell:
Two green-orange colocalization/fusion signals (2GO).

Expected Pattern 2

Aberrant Cell (typical results):
One green-orange colocalization/fusion signal (1GO), one separate green (1G) and orange (1O) signal each resulting from a chromosome break in the relevant locus.

Literature

  • Michaux et al (1996) Genes Chrom Can 15:38-47
  • Huh et al (2011) Am J Clin Pathol 135:686-696
  • Puiggros et al (2014) Biomed Res Int 2014:Article ID 435983

Downloads

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