XL BCL3 BA consists of an orange-labeled probe hybridizing proximal to the BCL3 gene region at 19q13.3 and a green-labeled probe hybridizing distal to the BCL3 gene region at 19q13.3.

Probe maps are created in accordance with the intended purpose of the product. Solid colored bars do not necessarily indicate that the probe fully covers the indicated genomic region. Therefore, caution is advised when interpreting results generated through off-label use. Probe map details based on UCSC Genome Browser GRCh37/hg19. Map components not to scale. Further information is available on request.

Chronic lymphocytic leukemia (CLL) is the most common leukemia in adults. The clinical course is heterogeneous and ranges from good outcome to very aggressive and fast progressing disease. CLL is not characterized by a well-defined chromosomal translocation as many other lymphoid neoplasms. The most frequent aberrations are deletions on 6q21 (3-6%), 11q22-23 (5-20%), 13q14.3 (>50%) or 17q13.1 (3-8%) and trisomy 12 (10-20%).
The recurrent t(14;19)(q32.3;q13.3) is a rare event with an incidence of <0.1% in B-cell neoplasms and is often associated with trisomy 12 or a complex karyotype. It is also considered as a poor prognostic marker in CLL with inferior outcome. The translocation juxtaposes BCL3 with the immunoglobulin heavy chain gene region on chromosome 14, resulting in overexpression of BCL3. BCL3 is an oncogene involved in the regulation of NF-kappa-B target genes.

Since it is difficult to obtain metaphases from CLL patients, interphase FISH offers great advantage over conventional cytogenetics.

Clinical Applications

• Chronic Lymphocytic Leukemia (CLL)

XL BCL3 BA hybridized to bone marrow cells. One aberrant cell with split signal is shown, indicating a break in the BCL3 locus.

• Michaux et al (1996) Genes Chrom Can 15:38-47
• Huh et al (2011) Am J Clin Pathol 135:686-696
• Puiggros et al (2014) Biomed Res Int 2014:Article ID 435983

• Utility and performance of bacterial artificial chromosomes-on-beads assays in chromosome analysis of clinical prenatal samples, products of conception and blood samples.
• A child with multiple congenital anomalies due to partial trisomy 7q22.1 → qter resulting from a maternally inherited balanced translocation: a case report and review of literature.
• Distinct roles of ATM and ATR in the regulation of ARP8 phosphorylation to prevent chromosome translocations.
• Heterogeneous MYCN amplification in neuroblastoma: a SIOP Europe Neuroblastoma Study.
• Immense random colocalization, revealed by automated high content image cytometry, seriously questions FISH as gold standard for detecting EML4-ALK fusion.
• Fluorescence in situ hybridisation assays designed for del(7q) detection uncover more complex rearrangements in myeloid leukaemia cell lines
• Detection of t(7;12)(q36;p13) in paediatric leukaemia using dual colour fluorescence in situ hybridisation.
• A rare case of t(11;22) in a mantle cell lymphoma like B-cell neoplasiaresulting in a fusion of IGL and CCND1: case report.
• Genomic DNA-chip hybridization in t(11;14)-positive mantle cell lymphomas shows a high frequency of aberrations and allows a refined characterization of consensus regions.