XL TLX3 BA

Break Apart Probe

Order Number
D-5129-100-OG
Package Size
100 µl
Labels
  
Chromosome
5

Description

XL TLX3 BA

XL TLX3 BA is designed as a break apart probe. The orange labeled probe hybridizes proximal to the breakpoint in the TLX3 gene region at 5q35, the green labeled probe hybridizes distal to the breakpoint.

Clinical Details

Acute lymphoblastic leukemia (ALL) is the most common childhood cancer type. T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive and quickly progressing type of ALL affecting T-lymphocytes. Genomic data suggests that more than 10 functional aberrations are contributing to the development of this disease. T-ALL cases can be grouped by distinct genetic profiles and the aberrant expression of a characteristic transcription factor. Major subgroups are characterized by ectopic expression of TAL1, TLX1, TLX3, HOXA9/10, LMO2 or NKX2-1 and others as a result of chromosomal rearrangements or mutations. About 20 % of childhood T-ALL cases are characterized by aberrant expression of TLX3 as a result of t(5;14)(q35;q32). This cryptic translocation juxtaposes TLX3, normally not expressed in T-cells, with the BCL11B gene which is active in T-cells and results in ectopic expression of TLX3. Fluorescence in situ hybridization is a valuable method for the detection of t(5;14)(q35;q32) since cryptic translocations may escape during classical cytogenetic analysis. Furthermore, the broad range of breakpoints in the chromosomal region 14q32 makes the development of efficient PCR-based methods difficult.

Clinical Applications

  • Acute Lymphoblastic Leukemia (ALL)

Images

XL TLX3 BA

XL TLX3 BA hybridized to lymphocytes. One normal interphase and metaphase are shown.

Expected Patterns

Expected Pattern 1

Normal Cell:
Two green-orange colocalization/fusion signals (2GO).

Expected Pattern 2

Aberrant Cell (typical results):
One green-orange colocalization/fusion signal (1GO), one separate green (1G) and orange (1O) signal each resulting from a chromosome break in the relevant locus.

Literature

  • Van Zutven et al (2004) Haematologica 89:671-678
  • Su et al (2006) Blood 108:4198-4201
  • Girardi et al (2017) Blood 129:1113-1123

Downloads

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