XL ALK BA

Break Apart Probe

Order Number
D-6001-100-OG
Package Size
100 µl
Labels
  
Chromosome
2

Description

XL ALK BA

XL ALK BA is a break apart probe with an orange labeled probe hybridizing to the proximal region within ALK in 2p23 and a green labeled probe hybridizing distal to ALK.

This probe is intended for methanol/acetic-acid fixed cells and tissue sections.

Clinical Details

The EML4/ALK fusion gene is responsible for approximately 3-5 % of non-small-cell lung cancer (NSCLC). The vast majority of cases are adenocarcinomas. Two other fusion partners of ALK have been reported in lung cancer - TFG and KIF5B. Up to 12 other translocation partners have been described in anaplastic large-cell lymphomas (ACLC), renal cancer, esophageal squamous cell, colon, and breast carcinoma. Anaplastic lymphoma kinase (ALK) is a receptor tyrosine kinase that was discovered in anaplastic large-cell lymphoma (ALC).

The formation of a fusion protein with a partner through chromosomal translocations is the most common mechanism of ALK overexpression and ALK kinase domain activation. The ALK inhibitor crizotinib has increased the significance of detecting ALK rearrangements in lung cancer and ALCL.

Clinical Applications

  • Solid Tumors (Solid Tumors)

Images

XL ALK BA

XL ALK BA hybridized on lung cancer tissue. Separated green and orange signals indicate an ALK rearrangement.

Expected Patterns

Expected Pattern 1

Normal Cell:
Two green-orange colocalization/fusion signals (2GO).

Expected Pattern 2

Aberrant Cell (typical results):
One green-orange colocalization/fusion signal (1GO), one separate green (1G) and orange (1O) signal each resulting from a chromosome break in the relevant locus.

Expected Pattern 3

Aberrant Cell (typical results):
Two green (2G) and one orange (1O) signal resulting from loss of one orange signal.

Literature

  • Kutok et al (2002) J Clin Oncol 20:3691-3702
  • Perner et al (2008) Neoplasia 10:298-302
  • Shaw et al (2009) J Clin Oncol 27:4247-4253

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