XL EGFR amp

Amplification Probe

Order Number
D-6005-100-OG
Package Size
100 µl
Labels
  
Chromosome
7

Description

XL EGFR amp

The XL EGFR amp probe detects amplifications in the short arm of chromosome 7. The orange labeled probe hybridizes to the EGFR locus at 7p11. A green labeled probe hybridizes to the 7cen region.

This probe is intended for methanol/acetic-acid fixed cells and tissue sections.

Clinical Details

EGFR (epidermal growth factor receptor) gene amplification generally results in increased protein expression in breast carcinomas. About 6 % of breast carcinomas show moderate- to low-level EGFR amplification associated with genuine EGFR protein overexpression. Studies in non-small cell lung cancer (NSCLC) have shown that EGFR expression is associated with reduced survival, frequent lymph node metastasis, and poor chemosensitivity.

EGFR is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases which all play an important role in controlling normal cell growth, apoptosis, and other cellular functions. Mutations of EGFRs can lead to non-small-cell lung cancer, pancreatic cancer, breast cancer, colon cancer, and some other cancers.

New drugs such as gefitinib and erlotinib directly target the EGFR. EGFR-positive patients have shown a 60 % response rate, which exceeds the response rate for conventional chemotherapy.

Clinical Applications

  • Solid Tumors (Solid Tumors)

Images

XL EGFR amp

XL EGFR amp hybridized to normal cells. Two green and two orange signals in each are shown.

Expected Patterns

Expected Pattern 1

Normal Cell:
Two green (2G) and two orange (2O) signals.

Expected Pattern 2

Aberrant Cell (typical results):
Two green (2G), one orange (1O), and orange signal clusters, indicating amplification of EGFR (homogenous staining region = HSR).

Expected Pattern 3

Aberrant Cell (typical results):
Two green (2G) and multiple copies of orange signals indicating amplification of EGFR (double minutes = dm).

Literature

  • Okada et al (2003) Cancer Res 63:413-416
  • Bhargava et al (2006) Mod Patho 18:1027-1033
  • Sholl et al (2009) Cancer Res 69:8341-8348

Downloads

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