Fluorescence in situ hybridization has become an essential detection assay in today´s routine diagnostics. However, long hybridization times of many hours to overnight are still a restrictive factor. We have refined the production process of our FISH probes to reduce background and artefacts and to improve the signal to noise ratio, particularly in short-time hybridization. Since mid-2015, one hour hybridization on lymphocytes is an integral part of quality control for all XCyting locus-specific probes at our manufacturing facility.
XL MYC amp
- Order Number
- Package Size
- 100 µl
The XL MYC amp probe detects amplifications in the long arm of chromosome 8. The orange labeled probe is designed to hybridize to the MYC locus at 8q24. A green labeled probe hybridizes to the 8cen region and functions as a control probe.
This probe is intended for methanol/acetic-acid fixed cells and tissue sections.
Amplification of MYC has been described in many types of tumor, including breast, cervical and colon cancers, as well as in squamous cell carcinomas of the head and neck, myeloma, non-Hodgkin lymphoma, gastric adenocarcinomas and ovarian cancer. MYC is the most frequently amplified oncogene and the elevated expression of its gene product correlates with tumor aggression and poor clinical outcome.
The proto-oncogene MYC, located at 8q24.1, encodes a nuclear phosphoprotein transcription factor that has an integral role in a variety of cellular processes, such as cell cycle progression, proliferation, metabolism, adhesion, differentiation, and apoptosis.
- Solid Tumors (Solid Tumors)
Two green (2G) and two orange (2O) signals.
Aberrant Cell (typical results):
Two green (2G), one orange (1O), and orange signal clusters, indicating amplification of MYC (homogenous staining region = HSR).
Aberrant Cell (typical results): Two green (2G) and multiple copies of orange signals, indicating amplification of MYC (double minutes = dm).
- Rummukainen et al (2001) Mod Pathol 14:1030-1035
- Blancato et al (2004) Br J Cancer 90:1612-1619
- Singhi et al (2012) Mod Pathol 25:378-387