XL ERBB2 (HER2/NEU) amp

Amplification Probe

Order Number
D-6010-100-OG
Package Size
100 µl
Labels
  
Chromosome
17

Description

XL ERBB2 (HER2/NEU) amp

The XL ERBB2 (HER2/NEU) amp probe detects amplifications in the long arm of chromosome 17. The orange labeled probe is designed to hybridize to the ERBB2 (HER2/NEU) locus at 17q12. A green labeled probe hybridizes to the 17cen region and functions as a control probe.

This probe is intended for methanol/acetic-acid fixed cells and tissue sections.

Clinical Details

Amplification or over-expression of the ERBB2 (HER2/NEU) gene occurs in approximately 15 - 30 % of breast cancers. It is strongly associated with increased disease recurrence and a poor prognosis. Over-expression is also known to occur in ovarian, stomach, and aggressive forms of uterine cancer, such as uterine serous endometrial carcinoma.

ERBB2 located on the long arm of human chromosome 17 (17q12), is a member of the epidermal growth factor receptor (EGFR/ErbB) family which is composed of four plasma membrane-bound receptor tyrosine kinases. Signaling through the ErbB family of receptors promotes cell proliferation and opposes apoptosis.
ERBB2 is the target of the monoclonal antibody trastuzumab (marketed as Herceptin®).

Clinical Applications

  • Solid Tumors (Solid Tumors)

Images

XL ERBB2 (HER2/NEU) amp

XL ERBB2 (HER2/NEU) amp hybridized on breast cancer tissue. Multiple orange signals indicate amplification of ERBB2 (HER2/NEU).

Expected Patterns

Expected Pattern 1

Normal Cell:
Two green (2G) and two orange (2O) signals.

Expected Pattern 2

Aberrant Cell (typical results):
Two green (2G), one orange (1O), and orange signal clusters, indicating amplification of ERBB2 (homogenous staining region = HSR).

Expected Pattern 3

Aberrant Cell (typical results): Two green (2G) and multiple copies of orange signals, indicating amplification of ERBB2 (double minutes = dm).

Literature

  • Isola et al (1999) Clin Cancer Res 5:4140-4145
  • Blancato et al (2004) Br J Cancer 90:1612-1619
  • Singhi et al (2012) Mod Pathol 25:378-387

Downloads

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