Fluorescence in situ hybridization has become an essential detection assay in today´s routine diagnostics. However, long hybridization times of many hours to overnight are still a restrictive factor. We have refined the production process of our FISH probes to reduce background and artefacts and to improve the signal to noise ratio, particularly in short-time hybridization. Since mid-2015, one hour hybridization on lymphocytes is an integral part of quality control for all XCyting locus-specific probes at our manufacturing facility.
XL EWSR1 BA
Break Apart Probe
- Order Number
- Package Size
- 100 µl
The Ewing sarcoma (EWS) is a rare and highly aggressive cancer with an incidence of about three in one millions Caucasians. The incidence in Africans is significantly lower. The EWS is more common among children and young adults than in adults and mostly arises in bones and to lower extend in soft tissue. EWS is typified by chromosomal translocations resulting in fusion genes between the EWS RNA Binding Protein 1 (EWSR1) and a member of the group of ETS transcription factors. t(11;22)(q24;q12) is the most common of these translocations represented by the EWSR1-FLI1 fusion gene with a frequency of about 85%. The EWSR1 part contributes a strong transcriptional domain while FLI1 is providing the ETS family DNA binding domain. The chimeric protein is dysregulating target genes leading to oncogenic transformation and is absolutely required for tumorigenesis in EWS. Other translocation partners are known, but no difference in survival has been observed between different fusion genes. Although complex karyotypes are rare in EWS, gain of chromosome 1q, 8, 12 and loss of 9p21 (CDKN2A) and 16q is observed. The EWSR gene is expressed in several tissues and is involved in other tumor disease. EWSR1 has manifold functions and is involved in different control mechanisms in the cell. EWS is genetically stable and complex karyotypes are rare.
- Solid Tumors (Solid Tumors)
Two green-orange colocalization/fusion signals (2GO).
Aberrant Cell (typical results):
One green-orange colocalization/fusion signal (1GO), one separate green (1G) and orange (1O) signal each resulting from a chromosome break in the respective locus.
- Delattre et al (1992) Nature 359:162-165
- Smith et al (2006) Cancer Cell 9:405-416
- Sannino et al (2017) Future Oncol. 13:1207-1211