XL BCL6 BA

Break Apart Probe

Order Number
D-6016-100-OG
Package Size
100 µl (10 Tests)
Labels
  
Chromosome
3

Description

XL BCL6 BA

XL BCL6 BA consists of a green-labeled probe hybridizing proximal to the BCL6 gene region at 3q27 and an orange-labeled probe hybridizing distal to the BCL6 gene region at 3q27-28.

Probe maps are created in accordance with the intended purpose of the product. Solid colored bars do not necessarily indicate that the probe fully covers the indicated genomic region. Therefore, caution is advised when interpreting results generated through off-label use. Probe map details based on UCSC Genome Browser GRCh37/hg19. Map components not to scale. Further information is available on request.

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Clinical Details

Follicular lymphoma (FL) and diffuse large B-cell lymphoma (DLBL) represent the two most common entities of non-Hodgkin lymphoma (NHL) worldwide with up to 22% and 31% of tumors, respectively. FLs have been classically associated with the translocation t(14;18)(q32;q21) in 70% to 95% of reported tumors, whereas translocations involving the BCL6 proto-oncogene locus in chromosomal band 3q27 are encountered in 6% to 14%. In DLBLs, a BCL6 translocation can be found in up to 40% of cases, while BCL2 translocations occur in 20% to 30%.

The relatively wide separation of the probe binding sites allows for the detection of translocations in the MBR (major breakpoint cluster region) as well as in the ABR (alternative breakpoint cluster region).

Clinical Applications

  • Non-Hodgkin Lymphomas (NHL)
  • Solid Tumors (Solid Tumors)
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Images

XL BCL6 BA

XL BCL6 BA hybridized to a positive case. The cell to the left shows one normal orange green fusion signal and one separated orange and green signal, indicating a break in BCL6.

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Expected Patterns

Expected Pattern 1

Normal Cell:
Two green-orange (2GO) fusion signals.

Expected Pattern 2

Aberrant Cell (typical results):
One green (1G), one orange (1O), and one green-orange (1GO) fusion signal, indicating a chromosome break in the BCL6 locus.

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Literature

  • Butler et al (2002) Cancer Res 62:4089-4094
  • Katzenberger et al (2004) Am J Pathol 165:481-490
  • Iqbal et al (2007) Leukemia 21:2332-2343

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