The meeting of the German Society of Human Genetics (gfh) was held in Weimar, Germany this week and of course the attendees had the opportunity to visit the joined booth of MetaSystems and MetaSystems Probes. Main topics of the exhibition have been the latest advancements of the Neon case and imaging platform and RapidHyb, the revolutionary fast hybridization times of MetaSystems Probes' FISH probes.
XL BCL6 BA
Break Apart Probe
- Order Number
- Package Size
- 100 µl (10 Tests)
The XL BCL6 BA probe is designed as a break apart probe. Its green labeled part hybridizes proximal to the BCL6 gene at 3q27, the orange labeled probe hybridizes to the region distal of BCL6.
This probe is intended for methanol/acetic-acid fixed cells and tissue sections.
Probe map details based on UCSC Genome Browser GRCh37/hg19. Map components not to scale.
Follicular lymphoma (FL) and diffuse large B-cell lymphoma (DLBL) represent the two most common entities of non-Hodgkin lymphoma (NHL) worldwide with up to 22% and 31% of tumors, respectively. FLs have been classically associated with the translocation t(14;18)(q32;q21) in 70% to 95% of reported tumors, whereas translocations involving the BCL6 proto-oncogene locus in chromosomal band 3q27 are encountered in 6% to 14%. In DLBLs a BCL6 translocation can be found in up to 40% of cases, while BCL2 translocations occur in 20% to 30%.
The relative wide separation of the probe binding sites allows for the detection of translocations in the MBR (major breakpoint cluster region) as well as in the ABR (alternative breakpoint cluster region).
- Non-Hodgkin Lymphomas (NHL)
- Solid Tumors (Solid Tumors)
Two green-orange (2GO) fusion signals.
Aberrant Cell (typical results):
One green (1G), one orange (1O), and one green-orange (1GO) fusion signal, indicating a chromosome break in the BCL6 locus.
- Butler et al (2002) Cancer Res 62: 4089-4094
- Katzenberger et al (2004) Am J Pathol 165: 481-490
- Iqbal et al (2007) Leukemia 21: 2332-2343