Fluorescence in situ hybridization has become an essential detection assay in today´s routine diagnostics. However, long hybridization times of many hours to overnight are still a restrictive factor. We have refined the production process of our FISH probes to reduce background and artefacts and to improve the signal to noise ratio, particularly in short-time hybridization. Since mid-2015, one hour hybridization on lymphocytes is an integral part of quality control for all XCyting locus-specific probes at our manufacturing facility.
XL BCL6 BA
Break Apart Probe
- Order Number
- Package Size
- 100 µl
Follicular lymphoma (FL) and diffuse large B-cell lymphoma (DLBL) represent the two most common entities of non-Hodgkin lymphoma (NHL) worldwide with up to 22% and 31% of tumors, respectively. FLs have been classically associated with the translocation t(14;18)(q32;q21) in 70% to 95% of reported tumors, whereas translocations involving the BCL6 proto-oncogene locus in chromosomal band 3q27 are encountered in 6% to 14%. In DLBLs a BCL6 translocation can be found in up to 40% of cases, while BCL2 translocations occur in 20% to 30%.
The relative wide separation of the probe binding sites allows for the detection of translocations in the MBR (major breakpoint cluster region) as well as in the ABR (alternative breakpoint cluster region).
- Non-Hodgkin Lymphomas (NHL)
- Solid Tumors (Solid Tumors)
Two green-orange (2GO) fusion signals.
Aberrant Cell (typical results):
One green (1G), one orange (1O), and one green-orange (1GO) fusion signal, indicating a chromosome break in the BCL6 locus.
- Butler et al (2002) Cancer Res 62: 4089-4094
- Katzenberger et al (2004) Am J Pathol 165: 481-490
- Iqbal et al (2007) Leukemia 21: 2332-2343