XL BCL6 BA consists of a green-labeled probe hybridizing proximal to the BCL6 gene region at 3q27 and an orange-labeled probe hybridizing distal to the BCL6 gene region at 3q27-28.

Probe maps are created in accordance with the intended purpose of the product. Solid colored bars do not necessarily indicate that the probe fully covers the indicated genomic region. Therefore, caution is advised when interpreting results generated through off-label use. Probe map details based on UCSC Genome Browser GRCh37/hg19. Map components not to scale. Further information is available on request.

Follicular lymphoma (FL) and diffuse large B-cell lymphoma (DLBL) represent the two most common entities of non-Hodgkin lymphoma (NHL) worldwide with up to 22% and 31% of tumors, respectively. FLs have been classically associated with the translocation t(14;18)(q32;q21) in 70% to 95% of reported tumors, whereas translocations involving the BCL6 proto-oncogene locus in chromosomal band 3q27 are encountered in 6% to 14%. In DLBLs, a BCL6 translocation can be found in up to 40% of cases, while BCL2 translocations occur in 20% to 30%.

The relatively wide separation of the probe binding sites allows for the detection of translocations in the MBR (major breakpoint cluster region) as well as in the ABR (alternative breakpoint cluster region).

Clinical Applications

• Non-Hodgkin Lymphomas (NHL)
• Solid Tumors (Solid Tumors)

XL BCL6 BA hybridized to a positive case. The cell to the left shows one normal orange green fusion signal and one separated orange and green signal, indicating a break in BCL6.

• Butler et al (2002) Cancer Res 62:4089-4094
• Katzenberger et al (2004) Am J Pathol 165:481-490
• Iqbal et al (2007) Leukemia 21:2332-2343

• Utility and performance of bacterial artificial chromosomes-on-beads assays in chromosome analysis of clinical prenatal samples, products of conception and blood samples.
• A child with multiple congenital anomalies due to partial trisomy 7q22.1 → qter resulting from a maternally inherited balanced translocation: a case report and review of literature.
• Distinct roles of ATM and ATR in the regulation of ARP8 phosphorylation to prevent chromosome translocations.
• Heterogeneous MYCN amplification in neuroblastoma: a SIOP Europe Neuroblastoma Study.
• Immense random colocalization, revealed by automated high content image cytometry, seriously questions FISH as gold standard for detecting EML4-ALK fusion.
• Fluorescence in situ hybridisation assays designed for del(7q) detection uncover more complex rearrangements in myeloid leukaemia cell lines
• Detection of t(7;12)(q36;p13) in paediatric leukaemia using dual colour fluorescence in situ hybridisation.
• A rare case of t(11;22) in a mantle cell lymphoma like B-cell neoplasiaresulting in a fusion of IGL and CCND1: case report.
• Genomic DNA-chip hybridization in t(11;14)-positive mantle cell lymphomas shows a high frequency of aberrations and allows a refined characterization of consensus regions.