Fluorescence in situ hybridization has become an essential detection assay in today´s routine diagnostics. However, long hybridization times of many hours to overnight are still a restrictive factor. We have refined the production process of our FISH probes to reduce background and artefacts and to improve the signal to noise ratio, particularly in short-time hybridization. Since mid-2015, one hour hybridization on lymphocytes is an integral part of quality control for all XCyting locus-specific probes at our manufacturing facility.
XL BCL2 BA
Break Apart Probe
- Order Number
- Package Size
- 100 µl
The BCL2 gene rearrangement can be found in 50 % of follicular lymphoma, 23.3 % of B-cell lymphoma, and about 15 % of diffuse large B-cell lymphoma. A consequence of this translocation is an overexpression of anti-apoptotic protein BCL2, which most likely represents the initial step of malignant transformation.
The majority of rearrangements in BCL2 occur at two distinct chromosomal regions, the major breakpoint cluster region (MBR) in 70 % and the minor cluster region in 10 % of patient's tumors. FISH has been shown to be of higher sensitivity and of equivalent specificity when compared to PCR on paraffin-embedded tissue sections.
- Non-Hodgkin Lymphomas (NHL)
- Solid Tumors (Solid Tumors)
Two green-orange (2GO) fusion signals representing the two normal BCL2 loci.
Aberrant Cell (typical results):
One green (1G), one orange (1O), and one green-orange (1GO) fusion signal, indicating a chromosome break in the BCL2 locus.
- Vaandrager et al (2000) Blood 96:1947-1952
- Godon et al (2003) Leukemia 17:255-259
- Weinberg et al (2007) J Mol Diagn 9:530-537