About 100 guests from 36 countries met on the XVIII. MetaSystems Distributor Meeting (DM) in November to exchange experiences and to get to know new trends and developments at MetaSystems.
XL BCL2 BA
Break Apart Probe
- Order Number
- Package Size
- 100 µl (10 Tests)
XL BCL2 BA consists of an orange-labeled probe hybridizing proximal to the BCL2 gene region at 18q21.3 and a green-labeled probe hybridizing distal to the BCL2 gene region at 18q21.3.
Probe maps are created in accordance with the intended purpose of the product. Solid colored bars do not necessarily indicate that the probe fully covers the indicated genomic region. Therefore, caution is advised when interpreting results generated through off-label use. Probe map details based on UCSC Genome Browser GRCh37/hg19. Map components not to scale. Further information is available on request.
The BCL2 gene rearrangement can be found in 50% of follicular lymphoma, 23.3% of B-cell lymphoma, and about 15% of diffuse large B-cell lymphoma. A consequence of this translocation is an overexpression of anti-apoptotic protein BCL2, which most likely represents the initial step of malignant transformation.
The majority of rearrangements in BCL2 occur at two distinct chromosomal regions, the major breakpoint cluster region (MBR) in 70% and the minor cluster region in 10% of patient's tumors.
FISH has been shown to be of higher sensitivity and of equivalent specificity when compared to PCR on paraffin-embedded tissue sections.
- Non-Hodgkin Lymphomas (NHL)
- Solid Tumors (Solid Tumors)
Two green-orange (2GO) fusion signals representing the two normal BCL2 loci.
Aberrant Cell (typical results):
One green (1G), one orange (1O), and one green-orange (1GO) fusion signal, indicating a chromosome break in the BCL2 locus.
- Vaandrager et al (2000) Blood 96:1947-1952
- Godon et al (2003) Leukemia 17:255-259
- Weinberg et al (2007) J Mol Diagn 9:530-537