XL BCL2 BA

Break Apart Probe

Order Number
D-6018-100-OG
Package Size
100 µl
Labels
  
Chromosome
18

Description

XL BCL2 BA

The XL BCL2 BA probe is designed as a break apart probe. The orange labeled part hybridizes proximal to the breakpoint in the BCL2 gene region at 18q21, the green labeled probe hybridizes distal to the breakpoint.

This probe is intended for methanol/acetic-acid fixed cells and tissue sections.

Clinical Details

The BCL2 gene rearrangement can be found in 50 % of follicular lymphoma, 23.3 % of B-cell lymphoma, and about 15 % of diffuse large B-cell lymphoma. A consequence of this translocation is an overexpression of anti-apoptotic protein BCL2, which most likely represents the initial step of malignant transformation.

The majority of rearrangements in BCL2 occur at two distinct chromosomal regions, the major breakpoint cluster region (MBR) in 70 % and the minor cluster region in 10 % of patient's tumors. FISH has been shown to be of higher sensitivity and of equivalent specificity when compared to PCR on paraffin-embedded tissue sections.

Clinical Applications

  • Non-Hodgkin Lymphomas (NHL)
  • Solid Tumors (Solid Tumors)

Images

XL BCL2 BA

XL BCL2 BA hybridized to a prostate tissue section. The normal green-orange fusion signal pattern is observed.

Expected Patterns

Expected Pattern 1

Normal Cell:
Two green-orange (2GO) fusion signals representing the two normal BCL2 loci.

Expected Pattern 2

Aberrant Cell (typical results):
One green (1G), one orange (1O), and one green-orange (1GO) fusion signal, indicating a chromosome break in the BCL2 locus.

Literature

  • Vaandrager et al (2000) Blood 96:1947-1952
  • Godon et al (2003) Leukemia 17:255-259
  • Weinberg et al (2007) J Mol Diagn 9:530-537

Downloads

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