Fluorescence in situ hybridization has become an essential detection assay in today´s routine diagnostics. However, long hybridization times of many hours to overnight are still a restrictive factor. We have refined the production process of our FISH probes to reduce background and artefacts and to improve the signal to noise ratio, particularly in short-time hybridization. Since mid-2015, one hour hybridization on lymphocytes is an integral part of quality control for all XCyting locus-specific probes at our manufacturing facility.
XL MYC BA
Break Apart Probe
- Order Number
- Package Size
- 100 µl
Translocations involving MYC are observed in diffuse large-B-cell lymphoma, follicular lymphoma, mantle cell lymphoma, and other lymphomas. In Burkitt Lymphoma the MYC gene, located at 8q24, is activated by a translocation next to an immunoglobulin constant gene. Most frequently MYC is positioned near the immunoglobulin heavy-chain (IGH) constant region on chromosome 14q32. However in some tumors, MYC can also be positioned near the light-chain region on chromosome 2p11 (IGK) or 22q11 (IGL). In addtion other translocation partners have been identified (e.g. BCL11A, PAX5, ZCCHC7).
The XL MYC probe is designed as a break apart probe with two probes juxtaposed and differently labeled. The proximal and distal region is sufficiently large to achieve intense signals also on paraffin-embedded tissue sections. The wide gap between the orange and green part of this probe allows for the detection of the t(2;8) translocation as well as t(8;14) and t(8;22).
- Non-Hodgkin Lymphomas (NHL)
- Solid Tumors (Solid Tumors)
Two green-orange (2GO) fusion signals.
Aberrant Cell (typical results):
One green (1G), one orange (1O), and one green-orange (1GO) fusion signal, indicating a chromosome break in the MYC locus.
- Hummel et al (2006) N Engl J Med 354: 2419-2430
- Einerson et al (2006) Leukemia 20: 1790-1799
- Bertrand et al (2007) Leukemia 21: 515-523