XL MYC BA

Break Apart Probe

Order Number
D-6023-100-OG
Package Size
100 µl (10 Tests)
Labels
  
Chromosome
8

Description

XL MYC BA

XL MYC BA consists of an orange-labeled probe hybridizing proximal to the MYC gene region at 8q24.21 and a green-labeled probe hybridizing distal to the MYC gene region at 8q24.21.

Probe maps are created in accordance with the intended purpose of the product. Solid colored bars do not necessarily indicate that the probe fully covers the indicated genomic region. Therefore, caution is advised when interpreting results generated through off-label use. Probe map details based on UCSC Genome Browser GRCh37/hg19. Map components not to scale. Further information is available on request.

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Clinical Details

Translocations involving MYC are observed in diffuse large-B-cell lymphoma, follicular lymphoma, mantle cell lymphoma, and other lymphomas. In Burkitt Lymphoma, the MYC gene, located at 8q24, is activated by a translocation next to an immunoglobulin constant gene. Most frequently, MYC is positioned near the immunoglobulin heavy-chain (IGH) constant region on chromosome 14q32. However, in some tumors MYC can also be positioned near the light-chain region on chromosome 2p11 (IGK) or 22q11 (IGL). In addtion, other translocation partners have been identified (e.g. BCL11A, PAX5, ZCCHC7).

The XL MYC probe is designed as a break apart probe with two probes juxtaposed and differentially labeled. The proximal and distal regions are sufficiently large to achieve intense signals also on paraffin-embedded tissue sections. The wide gap between the orange and green part of this probe allows for the detection of the t(2;8) translocation as well as t(8;14) and t(8;22).

Clinical Applications

  • Non-Hodgkin Lymphomas (NHL)
  • Solid Tumors (Solid Tumors)
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Images

XL MYC BA

XL MYC BA hybridized to lymphocytes. Separate orange and green signals indicate a split in the MYC gene region.

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Expected Patterns

Expected Pattern 1

Normal Cell:
Two green-orange (2GO) fusion signals.

Expected Pattern 2

Aberrant Cell (typical results):
One green (1G), one orange (1O), and one green-orange (1GO) fusion signal, indicating a chromosome break in the MYC locus.

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Literature

  • Hummel et al (2006) N Engl J Med 354:2419-2430
  • Einerson et al (2006) Leukemia 20:1790-1799
  • Bertrand et al (2007) Leukemia 21:515-523

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