XL MYC BA

Break Apart Probe

Order Number
D-6023-100-OG
Package Size
100 µl
Labels
  
Chromosome
8

Description

XL MYC BA

The XL MYC BA probe is designed as a break apart probe. The orange labeled probe hybridizes proximal to the breakpoint in the MYC gene region at 8q24, the green labeled probe hybridizes distal to the breakpoint.

This probe is intended for methanol/acetic-acid fixed cells and tissue sections.

Clinical Details

Translocations involving MYC are observed in diffuse large-B-cell lymphoma, follicular lymphoma, mantle cell lymphoma, and other lymphomas. In Burkitt Lymphoma the MYC gene, located at 8q24, is activated by a translocation next to an immunoglobulin constant gene. Most frequently MYC is positioned near the immunoglobulin heavy-chain (IGH) constant region on chromosome 14q32. However in some tumors, MYC can also be positioned near the light-chain region on chromosome 2p11 (IGK) or 22q11 (IGL). In addtion other translocation partners have been identified (e.g. BCL11A, PAX5, ZCCHC7).

The XL MYC probe is designed as a break apart probe with two probes juxtaposed and differently labeled. The proximal and distal region is sufficiently large to achieve intense signals also on paraffin-embedded tissue sections. The wide gap between the orange and green part of this probe allows for the detection of the t(2;8) translocation as well as t(8;14) and t(8;22).

Clinical Applications

  • Non-Hodgkin Lymphomas (NHL)
  • Solid Tumors (Solid Tumors)

Images

XL MYC BA

XL MYC BA hybridized to lymphocytes. Separate orange and green signals indicate a split in the MYC gene region.

Expected Patterns

Expected Pattern 1

Normal Cell:
Two green-orange (2GO) fusion signals.

Expected Pattern 2

Aberrant Cell (typical results):
One green (1G), one orange (1O), and one green-orange (1GO) fusion signal, indicating a chromosome break in the MYC locus.

Literature

  • Hummel et al (2006) N Engl J Med 354: 2419-2430
  • Einerson et al (2006) Leukemia 20: 1790-1799
  • Bertrand et al (2007) Leukemia 21: 515-523

Downloads

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