XL ROS1-GOPC BA

Break Apart Probe

Order Number
D-6029-100-OG
Package Size
100 µl (10 Tests)
Labels
  
Chromosome
6

Description

XL ROS1-GOPC BA

XL ROS1-GOPC BA consists of a green-labeled probe hybridizing proximal to the ROS1 gene region at 6q22.1 and an orange-labeled probe hybridizing distal to the ROS1 gene region at 6q22.1.

Probe maps are created in accordance with the intended purpose of the product. Solid colored bars do not necessarily indicate that the probe fully covers the indicated genomic region. Therefore, caution is advised when interpreting results generated through off-label use. Probe map details based on UCSC Genome Browser GRCh37/hg19. Map components not to scale. Further information is available on request.

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Clinical Details

Translocations involving the ROS1 receptor tyrosine kinase gene have recently been described in a subset of non-small-cell lung cancers (NSCLCs). Chromosomal rearrangements involving the ROS1 gene were originally described in glioblastomas, where ROS1 is fused to the GOPC gene by an interstitial deletion.
Fusions of ROS1 with other genes lead to constitutive kinase activity and are associated with in vitro sensitivity to tyrosine kinase inhibitors such as crizotinib.

Clinical Applications

  • Solid Tumors (Solid Tumors)
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Images

XL ROS1-GOPC BA

XL ROS1-GOPC BA hybridized to the U-138 cell line. Several interphase nuclei show the expected fusion pattern, while one cell to the left is showing a homozygous deletion of the orange signals.

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Expected Patterns

Expected Pattern 1

Normal Cell:
Two green-orange (2GO) fusion signals representing the two normal ROS1-GOPC loci.

Expected Pattern 2

Aberrant Cell (typical results):
One green (1G), one orange (1O), and one green-orange (1GO) fusion signal, indicating a chromosome break in the ROS1-GOPC locus.

Expected Pattern 3

Aberrant Cell (typical results):
One green (1G) and one green-orange (1GO) fusion signal, indicating a rearrangement of ROS1 by a deletion beetween ROS1 and GOPC.

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Literature

  • Charest et al (2003) Genes Chrom Cancer 37:58-71
  • Takeuchi et al (2011) Nat Med 18:378-381
  • Bergethon et al (2012) J Clin Oncol 30:863-870

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