XL ROS1-GOPC BA

Break Apart Probe

Order Number
D-6029-100-OG
Package Size
100 µl
Labels
  
Chromosome
6

Description

XL ROS1-GOPC BA

The XL ROS1-GOPC BA probe is designed as a break apart probe. Its green labeled part hybridizes proximal to the ROS1 gene at 6q22 and the orange labeled probe hybridizes to the distal region of ROS1. This probe is also suited to analyze the ROS1-GOPC (formerly called FIG-ROS) fusion, caused by an interstitial deletion of 240 kb in size.

This probe is intended for methanol/acetic-acid fixed cells and tissue sections.

Clinical Details

Translocations involving the ROS1 receptor tyrosine kinase gene have recently been described in a subset of non-small-cell lung cancers (NSCLCs). Chromosomal rearrangements involving the ROS1 gene were originally described in glioblastomas, where ROS1 is fused to the GOPC gene by an interstitial deletion.

Fusions of ROS1 with other genes lead to constitutive kinase activity and are associated with sensitivity in vitro to tyrosine kinase inhibitors such as Crizotinib.

Clinical Applications

  • Solid Tumors (Solid Tumors)

Images

XL ROS1-GOPC BA

XL ROS1-GOPC BA hybridized to the U-138 cell line. Several interphase nuclei show the expected fusion pattern, while one cell to the left is showing a homozygous deletion of the orange signals.

Expected Patterns

Expected Pattern 1

Normal Cell:
Two green-orange (2GO) fusion signals representing the two normal ROS1-GOPC loci.

Expected Pattern 2

Aberrant Cell (typical results):
One green (1G), one orange (1O), and one green-orange (1GO) fusion signal, indicating a chromosome break in the ROS1-GOPC locus.

Expected Pattern 3

Aberrant Cell (typical results):
One green (1G) and one green-orange (1GO) fusion signal, indicating a rearrangement of ROS1 by a deletion beetween ROS1 and GOPC.

Literature

  • Charest et al (2003) Genes Chrom Cancer 37:58-71
  • Takeuchi et al (2011) Nat Med 18:378-381
  • Bergethon et al (2012) J Clin Oncol 30:863-870

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