XL MYC BA Triple-color

Break Apart Probe - Triple Color

Order Number
D-6030-100-TC
Package Size
100 µl
Labels
   
Chromosome
8

Description

XL MYC BA Triple-color

The XL MYC BA Triple-color probe is designed as a triple-color break apart probe. Its orange labeled part hybridizes proximal to the MYC gene at 8q24, the green labeled probe hybridizes to the distal region of MYC, and a blue (aqua) labeled probe covers MYC and adjacent regions.

This probe is intended for methanol/acetic-acid fixed cells and tissue sections.

Clinical Details

Translocations involving MYC are observed in diffuse large-B-cell lymphoma, follicular lymphoma, mantle cell lymphoma, and other lymphomas. In Burkitt Lymphoma the MYC gene, located at 8q24, is activated by a translocation next to an immunoglobulin constant gene. Most frequently MYC is positioned near the immunoglobulin heavy-chain (IGH) constant region on chromosome 14q32. However in some tumors, MYC can also be positioned near the light-chain region on chromosome 2p11 (IGK) or 22q11 (IGL). In addtion other translocation partners have been identified (e.g. BCL11A, PAX5, ZCCHC7).

The XL MYC BA Triple-color probe is designed as a break apart probe with three probes juxtaposed and differently labeled. In cases with 8q24 rearrangment the co-localization patterns of orange-blue versus green-blue allows to distinguish different breakpoints in MYC translocations which can be an aid in diagnosis.

Clinical Applications

  • Non-Hodgkin Lymphomas (NHL)
  • Solid Tumors (Solid Tumors)

Images

XL MYC BA Triple-color

XL MYC BA triple-color hybridized to lymphocytes. One nucleus shows the normal two green-orange-blue signals, the other nucleus shows a separated green signal indicating a break distal to the MYC gene region.

Expected Patterns

Expected Pattern 1

Normal Cell:
Two blue-green-orange (2BGO) fusion signals.

Expected Pattern 2

Aberrant Cell (typical results):
One green-blue (1GB) signal and one separate orange (1O) signal from the translocated chromosome and one blue-green-orange (1BGO) fusion signal from the normal chromosome.

Expected Pattern 3

Aberrant Cell (typical results):
One orange-blue (1OB) signal and one separate green (1G) signal from the translocated chromosomes and one blue-green-orange (1BGO) fusion signal from the normal chromosome.

Literature

  • Hummel et al (2006) N Engl J Med 354: 2419-2430
  • Einerson et al (2006) Leukemia 20: 1790- 1799
  • Bertrand et al (2007) Leukemia 21: 515-523

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