XL MYC BA triple-color

Break Apart Probe - Triple Color

Order Number
D-6030-100-TC
Package Size
100 µl (10 Tests)
Labels
   
Chromosome
8

Description

XL MYC BA triple-color

XL MYC BA triple-color consists of an orange-labeled probe hybridizing proximal to the MYC gene region at 8q24.21, an aqua-labeled probe hybridizing to the MYC gene region at 8q24.21 and a green-labeled probe hybridizing distal to the MYC gene region at 8q24.21.

Probe maps are created in accordance with the intended purpose of the product. Solid colored bars do not necessarily indicate that the probe fully covers the indicated genomic region. Therefore, caution is advised when interpreting results generated through off-label use. Probe map details based on UCSC Genome Browser GRCh37/hg19. Map components not to scale. Further information is available on request.

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Clinical Details

Translocations involving MYC are observed in diffuse large-B-cell lymphoma, follicular lymphoma, mantle cell lymphoma, and other lymphomas. In Burkitt Lymphoma, the MYC gene, located at 8q24, is activated by a translocation next to an immunoglobulin constant gene. Most frequently, MYC is positioned near the immunoglobulin heavy-chain (IGH) constant region on chromosome 14q32. However, in some tumors MYC can also be positioned near the light-chain region on chromosome 2p11 (IGK) or 22q11 (IGL). In addtion, other translocation partners have been identified (e.g. BCL11A, PAX5, ZCCHC7).

The XL MYC BA Triple-color probe is designed as a break apart probe with three probes juxtaposed and differentially labeled. In cases with 8q24 rearrangment, the co-localization patterns of orange-blue versus green-blue allows to distinguish different breakpoints in MYC translocations which can be an aid in diagnosis.

Clinical Applications

  • Non-Hodgkin Lymphomas (NHL)
  • Solid Tumors (Solid Tumors)
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Images

XL MYC BA triple-color

XL MYC BA triple-color hybridized to lymphocytes. One nucleus shows the normal two green-orange-blue signals, the other nucleus shows a separated green signal indicating a break distal to the MYC gene region.

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Expected Patterns

Expected Pattern 1

Normal Cell:
Two blue-green-orange (2BGO) fusion signals.

Expected Pattern 2

Aberrant Cell (typical results):
One green-blue (1GB) signal and one separate orange (1O) signal from the translocated chromosome and one blue-green-orange (1BGO) fusion signal from the normal chromosome.

Expected Pattern 3

Aberrant Cell (typical results):
One orange-blue (1OB) signal and one separate green (1G) signal from the translocated chromosomes and one blue-green-orange (1BGO) fusion signal from the normal chromosome.

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Literature

  • Hummel et al (2006) N Engl J Med 354:2419-2430
  • Einerson et al (2006) Leukemia 20:1790-1799
  • Bertrand et al (2007) Leukemia 21:515-523

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