The meeting of the German Society of Human Genetics (gfh) was held in Weimar, Germany this week and of course the attendees had the opportunity to visit the joined booth of MetaSystems and MetaSystems Probes. Main topics of the exhibition have been the latest advancements of the Neon case and imaging platform and RapidHyb, the revolutionary fast hybridization times of MetaSystems Probes' FISH probes.
XL FOXO1 BA
Break Apart Probe
- Order Number
- Package Size
- 100 µl (10 Tests)
XL FOXO1 BA is designed as a break apart probe. The green labeled probe hybridizes proximal to the breakpoint in the FOXO1 gene region at 13q14, the orange labeled probe hybridizes distal to the breakpoint.
This probe is intended for methanol/acetic-acid fixed cells and tissue sections.
Probe map details based on UCSC Genome Browser GRCh37/hg19. Map components not to scale.
Rhabdomyosarcoma (RMS) is a relatively rare cancer type but it is the most common soft tissue sarcoma in children and adolescents. The histopathological classification includes several subtypes, the embryonal rhabdomyosarcoma (ERMS) and the alveolar rhabdomyosarcoma (ARMS) are the most common variants, in the order of their occurrence. ARMS is associated with a worse outcome and is characterized by the two reciprocal translocations t(2;13)(q35;q14) and t(1;13) (p36;q14), affecting the FOXO1 gene region and PAX3 or PAX7 respectively, in about 80% of cases. The result is an in-frame fusion of the PAX DNA binding domain with the transcriptional active domain of FOXO1 generating a highly potent chimeric activator for PAX target genes. Patients harboring FOXO1-PAX3/7 fusions have an inferior event free survival compared to patients without translocation, suggesting that the molecular status in RMS provides valuable prognostic information.
- Solid Tumors (Solid Tumors)
Two green-orange colocalization/fusion signals (2GO).
Aberrant Cell (typical results):
One green-orange colocalization/fusion signal (1GO), one separate green (1G) and orange (1O) signal each resulting from a chromosome break in the respective locus.
- McManus et al (1996) J Pathol 178:410–414
- Barr (2001) Oncogene 20:5736-5746
- Skapek et al (2013) Pediatr Blood Cancer 60:1411-1417