XL FOXO1 BA

Break Apart Probe

Order Number
D-6034-100-OG
Package Size
100 µl
Labels
  
Chromosome
13

Description

XL FOXO1 BA

XL FOXO1 BA is designed as a break apart probe. The green labeled probe hybridizes proximal to the breakpoint in the FOXO1 gene region at 13q14, the orange labeled probe hybridizes distal to the breakpoint.

This probe is intended for methanol/acetic-acid fixed cells and tissue sections.

Clinical Details

Rhabdomyosarcoma (RMS) is a relatively rare cancer type but it is the most common soft tissue sarcoma in children and adolescents. The histopathological classification includes several subtypes, the embryonal rhabdomyosarcoma (ERMS) and the alveolar rhabdomyosarcoma (ARMS) are the most common variants, in the order of their occurrence. ARMS is associated with a worse outcome and is characterized by the two reciprocal translocations t(2;13)(q35;q14) and t(1;13) (p36;q14), affecting the FOXO1 gene region and PAX3 or PAX7 respectively, in about 80% of cases. The result is an in-frame fusion of the PAX DNA binding domain with the transcriptional active domain of FOXO1 generating a highly potent chimeric activator for PAX target genes. Patients harboring FOXO1-PAX3/7 fusions have an inferior event free survival compared to patients without translocation, suggesting that the molecular status in RMS provides valuable prognostic information.

Clinical Applications

  • Solid Tumors (Solid Tumors)

Images

XL FOXO1 BA

XL FOXO1 hybridized to lymphocytes. Two normal interphases are shown.

Expected Patterns

Expected Pattern 1

Normal Cell:
Two green-orange colocalization/fusion signals (2GO).

Expected Pattern 2

Aberrant Cell (typical results):
One green-orange colocalization/fusion signal (1GO), one separate green (1G) and orange (1O) signal each resulting from a chromosome break in the respective locus.

Literature

  • McManus et al (1996) J Pathol 178:410–414
  • Barr (2001) Oncogene 20:5736-5746
  • Skapek et al (2013) Pediatr Blood Cancer 60:1411-1417

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