XL FUS BA

Break Apart Probe

Order Number
D-6035-100-OG
Package Size
100 µl
Labels
  
Chromosome
16

Description

XL FUS BA

XL FUS BA is designed as a break apart probe. The orange labeled probe hybridizes proximal to the breakpoint in the FUS gene region at 16p11, the green labeled probe hybridizes distal to the breakpoint.

This probe is intended for methanol/acetic-acid fixed cells and tissue sections.

Clinical Details

Myxoid liposarcomas (MLS) are accounting for about 30% of liposarcomas and represent approximately 10% of adult soft tissue sarcomas. Patients with MLS showing progression to round-cell morphology have an inferior outcome. The most common aberration in MLS is the translocation t(12;16)(q13;p11) with a frequency of about 95% and to a much lesser extend t(12;22)(q13;q12), in which FUS is not involved. These reciprocal translocations are resulting in the generation of FUS-DDIT3 and EWSR1-DDIT3 fusion genes, respectively. As FUS is also involved in the development of low-grade fibromyxoid sarcoma with the rearrangement t(7;16)(q33;p11), FUS rearrangements are not highly specific for the detection of MLS. Human models of sarcomagenesis suggest that the FUS-DDIT3 fusion gene impedes with adipogenic differentiation of mesenchymal stem cells and thereby contributes to the development of liposarcoma.

Clinical Applications

  • Solid Tumors (Solid Tumors)

Images

XL FUS BA

XL FUS BA hybridized to a tissue specimen, several aberrant cells are shown.

Expected Patterns

Expected Pattern 1

Normal Cell:
Two green-orange colocalization/fusion signals (2GO).

Expected Pattern 2

Aberrant Cell (typical results):
One green-orange colocalization/fusion signal (1GO), one separate green (1G) and orange (1O) signal each resulting from a chromosome break in the respective locus.

Literature

  • Knight et al (1995) Cancer Res 55:24-27
  • Tanas et al (2009) Adv Anat Pathol 16:383-391
  • Rodriguez et al (2013) Stem Cells 31:2061-2072

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