XL IRF4 BA

Break Apart Probe

Order Number
D-6040-100-OG
Package Size
100 µl
Labels
  
Chromosome
6

Description

XL IRF4 BA

The XL IRF4 BA probe is designed as a break apart probe. The orange labeled probe hybridizes distal to the breakpoint in the IRF4 gene region at 6p25, the green labeled probe hybridizes proximal to the breakpoint.

This probe is intended for methanol/acetic-acid fixed cells and tissue sections.

Clinical Details

The updated (2016) revision of the World Health Organization (WHO) classification of tumors of lymphoid neoplasms considers the large B-cell lymphoma with IRF4 rearrangement as a new provisional entity. Head and neck regions including the Waldeyer ring are particularly affected. Chromosomal translocations involving the IRF4 gene and the immunoglobulin loci IGH, IGL and IGK (IG) result in dysregulation of IRF4 expression, other translocation partners may occur. Since t(6;14)(p25;q32) is cytogenetically cryptic, FISH is a valuable tool in detecting this recurrent aberration. IG/IRF4 positive lymphomas normally lack t(14;18), observed in about 85% of adult patients with follicular lymphoma, and are commonly associated with young age and a good course. IRF4 rearrangements have also been identified in peripheral T-cell lymphomas (PTCL). PTCL are defined as a diverse group of aggressive lymphomas of mature-stage T-cells accounting for about 10% of Non-Hodgkin lymphomas. Recent data suggests, that IRF4 translocations detected by FISH have a predictive value for primary cutaneous CD30(+) anaplastic large cell lymphoma.

Clinical Applications

  • Non-Hodgkin Lymphomas (NHL)

Images

XL IRF4 BA

XL IRF4 BA hybridized to lymphozytes. One aberrant cell with split signal is shown, indicating a break in the IRF4 locus. Additionally, one normal interphase is shown.

Expected Patterns

Expected Pattern 1

Normal Cell:
Two green-orange colocalization/fusion signals (2GO).

Expected Pattern 2

Aberrant Cell (typical results):
One green-orange colocalization/fusion signal (1GO), one separate green (1G) and orange (1O) signal each resulting from a chromosome break in the relevant locus.

Literature

  • Feldman et al (2009) Leukemia 23:574-580
  • Salaverria et al (2011) Blood 7:139-147
  • Kiran et al (2013) Leukemia Research 37:396-400

Downloads

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