XL t(11;14) MYEOV/IGH DF

Translocation/Dual Fusion Probe

Order Number
D-5111-100-OG
Package Size
100 µl (10 Tests)
Labels
  
Chromosomes
1114

Description

XL t(11;14) MYEOV/IGH DF

XL t(11;14) MYEOV/IGH DF is designed as a dual fusion probe. The orange labeled probe hybridizes to gene region 11q13 (CCND1/MYEOV), the green labeled probe flanks the IGH breakpoint region at 14q32.

Probe map details based on UCSC Genome Browser GRCh37/hg19. Map components not to scale.

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Clinical Details

The mantle cell lymphoma (MCL) is a B-cell non-Hodgkin lymphoma with an aggressive clinical course. It is genetically characterized by t(11;14)(q13;q32) and is present in about 95% of MCL patients. By lower frequency, t(11;14) is also detectable in B-cell prolymphocytic leukemia, myelomas and chronic lymphocytic leukemia. The translocation induces overexpression of CCND1 which is normally not detected in B lymphocytes. CCND1 is a major player in cell cycle regulation and involved in the G1/S-phase transition. The oncogenic potential of CCND1 overexpression is related to its role in the cell cycle but also to other, non-cell cycle-related mechanisms as increased genomic instability and cell survival. t(11;14) is considered as a primary event, often followed by secondary chromosome alterations.
In multiple myeloma (MM), t(11;14) is the most common translocation, detectable in about 15-20% of all MM patients by FISH. Conventional cytogenetics has a much lower sensitivity, detecting t(11;14) in about 5% of MM patients. MM t(11;14) patients do have a relatively favorable outcome compared to other recurrent IGH translocations.

Clinical Applications

  • Non-Hodgkin Lymphomas (NHL)
  • Multiple Myeloma and Plasma Cell Neoplasms (MM)
  • Chronic Lymphocytic Leukemia (CLL)
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Images

XL t(11;14) MYEOV/IGH DF

XL t(11;14) IGH/MYEOV DF hybridized to lymphocytes. One normal metaphase and one normal interphase are shown.

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Expected Patterns

Expected Pattern 1

Normal Cell:
Two green (2G) and two orange (2O) signals.

Expected Pattern 2

Aberrant Cell (typical results):
One green (1G), one orange (1O), and two green-orange colocalization/fusion signals (2GO) resulting from a reciprocal translocation between the relevant loci.

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Literature

  • Fonesca et al (2002) Blood 99:3735-3741
  • Bentz et al (2004) Canc Cytopath 102:124-131
  • Jares et al (2012) J Clin Invest 122:3416-3423

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