The meeting of the German Society of Human Genetics (gfh) was held in Weimar, Germany this week and of course the attendees had the opportunity to visit the joined booth of MetaSystems and MetaSystems Probes. Main topics of the exhibition have been the latest advancements of the Neon case and imaging platform and RapidHyb, the revolutionary fast hybridization times of MetaSystems Probes' FISH probes.
XL BCL2 BA
Break Apart Probe
- Order Number
- Package Size
- 100 µl (10 Tests)
The XL BCL2 BA probe is designed as a break apart probe. The orange labeled part hybridizes proximal to the breakpoint in the BCL2 gene region at 18q21, the green labeled probe hybridizes distal to the breakpoint.
This probe is intended for methanol/acetic-acid fixed cells and tissue sections.
Probe map details based on UCSC Genome Browser GRCh37/hg19. Map components not to scale.
The BCL2 gene rearrangement can be found in 50 % of follicular lymphoma, 23.3 % of B-cell lymphoma, and about 15 % of diffuse large B-cell lymphoma. A consequence of this translocation is an overexpression of anti-apoptotic protein BCL2, which most likely represents the initial step of malignant transformation.
The majority of rearrangements in BCL2 occur at two distinct chromosomal regions, the major breakpoint cluster region (MBR) in 70 % and the minor cluster region in 10 % of patient's tumors. FISH has been shown to be of higher sensitivity and of equivalent specificity when compared to PCR on paraffin-embedded tissue sections.
- Non-Hodgkin Lymphomas (NHL)
- Solid Tumors (Solid Tumors)
Two green-orange (2GO) fusion signals representing the two normal BCL2 loci.
Aberrant Cell (typical results):
One green (1G), one orange (1O), and one green-orange (1GO) fusion signal, indicating a chromosome break in the BCL2 locus.
- Vaandrager et al (2000) Blood 96:1947-1952
- Godon et al (2003) Leukemia 17:255-259
- Weinberg et al (2007) J Mol Diagn 9:530-537