Fluorescence in situ hybridization has become an essential detection assay in today´s routine diagnostics. However, long hybridization times of many hours to overnight are still a restrictive factor. We have refined the production process of our FISH probes to reduce background and artefacts and to improve the signal to noise ratio, particularly in short-time hybridization. Since mid-2015, one hour hybridization on lymphocytes is an integral part of quality control for all XCyting locus-specific probes at our manufacturing facility.
Break Apart Probe
- Order Number
- Package Size
- 100 µl
Acute Lymphoblastic Leukemia (ALL) is the most common type of leukemia in children, representing almost 25 % of pediatric cancer. The majority of patients with ALL demonstrate an abnormal karyotype, either in chromosome number or as structural changes such as translocations, inversions, or deletions.
E2A (also termed TCF3) is the target of three known recurrent genomic rearrangements in ALL. The t(1;19)(q23;p13.3) occurs in approximately 5 % of cases and is the second most common translocation in ALL. The t(17;19)(q22;p13) occurs in about 1 % of ALLs and fuses E2A to the chromosome 17 gene HLF. A recently described cryptic inversion of chromosome 19 fuses E2A to the 19q13.4 gene FB1.
- Acute Lymphoblastic Leukemia (ALL)
Two green-orange fusion signals (2GO).
Aberrant Cell (typical results):
One green-orange fusion signal (1GO), one separate green (1G) and one orange (1O) signal each resulting from a chromosome break in the relevant locus.
- Boomer et al (2001) Leukemia 15:95-102
- Van der Burg et al (2004) Leukemia 18:895-908
- Kager et al (2007) Haematologica 92:1561-1564