Fluorescence in situ hybridization has become an essential detection assay in today´s routine diagnostics. However, long hybridization times of many hours to overnight are still a restrictive factor. We have refined the production process of our FISH probes to reduce background and artefacts and to improve the signal to noise ratio, particularly in short-time hybridization. Since mid-2015, one hour hybridization on lymphocytes is an integral part of quality control for all XCyting locus-specific probes at our manufacturing facility.
Break Apart Probe
- Order Number
- Package Size
- 100 µl
Several recurrent balanced translocations and inversions, and their variants, are
recognized in the WHO category acute myeloid leukemia (AML) with recurrent genetic abnormalities. Furthermore, several cytogenetic abnormalities are considered sufficient to establish the WHO diagnosis of AML with myelodysplasia-related features when 20% or more blood or marrow blasts are present.
The AML1 (RUNX1) gene on human chromosome 21q22.12 belongs to the 'runt domain' gene family of transcription factors. It is a key regulator of hematopoiesis and a frequent target of leukemia associated chromosomal translocations. RUNX1 can either activate or repress transcription of target genes, depending on the protein isoform, and is able to interact with other transcriptional regulators.
- Acute Lymphoblastic Leukemia (ALL)
- Acute Myelogenous Leukemia (AML)
Two green-orange (2GO) fusion signals representing the two normal AML1 loci.
Aberrant Cell (typical results):
One green (1G), one orange (1O), and one green-orange (1GO) fusion signals, indicating a chromosome break in the AML1 locus.
- Levanon et al (2001) Gene 262:23-33
- Zhang et al (2002) Proc Natl Acad Sci USA 99:3070-3075