Fluorescence in situ hybridization has become an essential detection assay in today´s routine diagnostics. However, long hybridization times of many hours to overnight are still a restrictive factor. We have refined the production process of our FISH probes to reduce background and artefacts and to improve the signal to noise ratio, particularly in short-time hybridization. Since mid-2015, one hour hybridization on lymphocytes is an integral part of quality control for all XCyting locus-specific probes at our manufacturing facility.
Break Apart Probe
- Order Number
- Package Size
- 100 µl
In 2008 the World Health Organization (WHO) classification of tumors of hematopoietic and lymphoid tissues introduced a new category for myeloid and lymphoid neoplasms with eosinophilia and abnormalities of PDGFRA, PDGFRB, or FGFR1. Many of these cases present as a myeloproliferative neoplasm, usually with eosinophilia.
Myeloid neoplasms (MPNs) with rearrangement of PDGFRB are phenotypically and genotypically diverse. The fusion genes involving PDGFRB described to date have been associated with cytogenetically detectable translocations. MPNs associated with rearrangement of PDGFRB are responsive to Imatinib.
- Chronic Myelogenous Leukemia and Myeloproliferative Neoplasms (CML/MPN)
Two green-orange fusion signals (2GO) representing the two normal PDGFRB loci.
Aberrant Cell (typical results):
One green-orange fusion signal (1GO) and one green (1G), one orange (1O) indicating a chromosome break in the PDGFRB locus.
- Wlodarska et al (1997) Blood 89:1716-1722
- Apperley et al (2002) N Engl J Med 347:481-487
- Wilkinson et al (2003) Blood 102:4187-4190