XL CLL contains different probe mixes provided in separate test vials.
XL DLEU/LAMP/12cen consists of a green-labeled probe hybridizing to the centromere of chromosome 12, an orange-labeled probe hybridizing to the DLEU1/MIR15A/MIR16-1 gene region at 13q14.2 including D13S319 and an aqua-labeled probe hybridizing to the LAMP1 gene region at 13q34.
XL ATM/TP53 consists of a green-labeled probe hybridizing to the ATM gene region at 11q22.3 and an orange-labeled probe hybridizing to the TP53 gene region at 17p13.

Probe maps are created in accordance with the intended purpose of the product. Solid colored bars do not necessarily indicate that the probe fully covers the indicated genomic region. Therefore, caution is advised when interpreting results generated through off-label use. Probe map details based on UCSC Genome Browser GRCh37/hg19. Map components not to scale. Further information is available on request.

The XL CLL Probe Kit is a combination of probes covering the most frequent chromosomal aberrations in chronic lymphocytic leukemia (CLL). For a more detailed description, please refer to product XL DLEU/LAMP/12cen (D-5055-100-TC) and XL ATM/TP53 (D-5046-100-OG).

Clinical Applications

• Chronic Lymphocytic Leukemia (CLL)
• Multiple Myeloma and Plasma Cell Neoplasms (MM)

The CLL kit hybridized to normal lymphocytes. The XL DLEU/LAMP/12cen probe (top) shows orange signals at 13q14, blue reference signals at 13q34, and green signals at the centromeric region of chromosome 12. The XL ATM/TP53 probe (bottom) shows orange signals at 17p13 and green signals at 11q22.

• Doehner et al (2000) N Engl J Med 343:1910-1916
• DeWald et al (2003) Brit J Haem 121:287-295
• Nelson et al (2007) Am J Clin Pathol 128:323-332

• Utility and performance of bacterial artificial chromosomes-on-beads assays in chromosome analysis of clinical prenatal samples, products of conception and blood samples.
• A child with multiple congenital anomalies due to partial trisomy 7q22.1 → qter resulting from a maternally inherited balanced translocation: a case report and review of literature.
• Distinct roles of ATM and ATR in the regulation of ARP8 phosphorylation to prevent chromosome translocations.
• Heterogeneous MYCN amplification in neuroblastoma: a SIOP Europe Neuroblastoma Study.
• Immense random colocalization, revealed by automated high content image cytometry, seriously questions FISH as gold standard for detecting EML4-ALK fusion.
• Fluorescence in situ hybridisation assays designed for del(7q) detection uncover more complex rearrangements in myeloid leukaemia cell lines
• Detection of t(7;12)(q36;p13) in paediatric leukaemia using dual colour fluorescence in situ hybridisation.
• A rare case of t(11;22) in a mantle cell lymphoma like B-cell neoplasiaresulting in a fusion of IGL and CCND1: case report.
• Genomic DNA-chip hybridization in t(11;14)-positive mantle cell lymphomas shows a high frequency of aberrations and allows a refined characterization of consensus regions.