XL MDM2

Amplification Probe

Order Number
D-5047-100-OG
Package Size
100 µl
Labels
  
Chromosome
12

Description

XL MDM2

The XL MDM2 hybridizes with an orange labeled probe to MDM2 and its flanking regions. A green labeled probe hybridizes to the centromere of chromosome 12.

Clinical Details

The tumor suppressor gene TP53 is a key regulator in the cell cycle and is involved in apoptosis, genomic stability and angiogenesis. Inactivation of TP53 function prevents the antiproliferative effect and contributes to the development of many types of cancer. Several mechanisms of TP53 loss of function are known as gene mutations, interaction of p53 with viral proteins and association of p53 with the MDM2 oncoprotein. MDM2 is an ubiquitin ligase negatively regulating p53. MDM2 is amplified in about 7% of all human cancers with the highest frequency of about 20% in soft tissue tumors. MDM2 might also have p53 independent transforming capabilities. Well-differentiated liposarcomas/atypical lipomatous tumors (WDL-ALT) and dedifferentiated liposarcomas are among the most common soft tissue tumors in adults. Both entities share the same genetic aberration, an amplification of the chromosomal region including MDM2. WDL-ALT and benign lipomatous tumors can be morphologically similar and a clear assignment based on histological features might be difficult. The analysis of the MDM2 amplification status by FISH is considered as a useful technique for the differential diagnosis of WDL-ALT and benign lipomatous tumors since benign lesions do not harbor MDM2 amplifications. Furthermore, MDM2 overexpression has been reported to be a potential cause of p53 dysfunction in Chronic lymphoblastic leukemia.

Clinical Applications

  • Chronic Lymphocytic Leukemia (CLL)
  • Solid Tumors (Solid Tumors)

Images

XL MDM2

XL MDM2 hybridized to lymphocytes. Two normal interphases and one normal metaphase are shown.

Expected Patterns

Expected Pattern 1

Normal Cell:
Two green (2G) and two orange (2O) signals.

Expected Pattern 2

Aberrant Cell (typical results):
Two green (2G) and three orange (3O) signals, indicating a duplication of the MDM2 locus.

Literature

  • Haidar et al (1997) Am J Hematol 54:189-195
  • Momand et al (1998) Nucleic Acids Res 26:3453-3459
  • Weaver et al (2008) Mod Pathol 21:943-949

Downloads

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